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Francesca VEZZALINI

Ricercatore Universitario
Dipartimento di Scienze della Vita sede ex-Scienze Biomediche

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Pubblicazioni

2019 - Cannabinoid profiling of hemp seed oil by liquid chromatography coupled to high-resolution mass spectrometry [Articolo su rivista]
Citti, Cinzia; Linciano, Pasquale; Panseri, Sara; Vezzalini, Francesca; Forni, Flavio; Vandelli, Maria Angela; Cannazza, Giuseppe
abstract

Hemp seed oil is well known for its nutraceutical, cosmetic and pharmaceutical properties due to a perfectly balanced content of omega 3 and omega 6 polyunsaturated fatty acids. Its importance for human health is reflected by the success on the market of organic goods in recent years. However, it is of utmost importance to consider that its healthy properties are strictly related to its chemical composition, which varies depending not only on the manufacturing method, but also on the hemp variety employed. In the present work, we analyzed the chemical profile of ten commercially available organic hemp seed oils. Their cannabinoid profile was evaluated by a liquid chromatography method coupled to high-resolution mass spectrometry. Besides tetrahydrocannabinol and cannabidiol, other 30 cannabinoids were identified for the first time in hemp seed oil. The results obtained were processed according to an untargeted metabolomics approach. The multivariate statistical analysis showed highly significant differences in the chemical composition and, in particular, in the cannabinoid content of the hemp oils under investigation.

2005 - Activity of single components of Ferula hermonis on male rat sexual behavior [Articolo su rivista]
Zanoli, Paola; Rivasi, Marianna; Zavatti, Manuela; F., Brusiani; Vezzalini, Francesca; Baraldi, Mario
abstract

The influence of the single components of Ferula hermonis extract on sexual behavior was studied in male rats. Sexually potent and sluggish/impotent animals were orally treated acutely (2.5 mg/kg) and subchronically (0.25 mg/kg/day for 10 days) with ferutinin, teferdin and teferin. Ferutinin alone acutely administered in potent rats was able to reduce mount and intromission latencies, while in sluggish/impotent animals, it induced the same effects and additionally shortened the ejaculation latency, as teferdin did. Both substances increased testosterone levels in rats. Unlike teferdin, ferutinin subchronically administered in potent rats negatively affected appetitive and consummatory sexual behavior, reducing also testosterone serum levels. In conclusion, if repetitively administered, ferutinin was able to stimulate sexual behavior after acute ingestion, but exerted a negative influence on the sexual capacity of potent male rats, whereas teferdin only improved copulatory performance of sluggish/impotent animals.

2005 - Synthesis and antiplatelet activity of thioaryloxyacids analogues of clofibric acid [Articolo su rivista]
A., Ammazzalorso; R., Amoroso; Baraldi, Mario; G., Bettoni; Braghiroli, Daniela; B., De Filippis; L., Giampietro; M. L., Tricca; Vezzalini, Francesca
abstract

The thiophene-, benzothiazole- and pyridine-thioaryloxyacids analogues of clofibric acid were synthesized and their antiplatelet activity was screened. Some compounds exhibited antiaggregating properties. The platelet-related haemostasis was measured on a PFA-100 (R) analyzer using bull blood.

2003 - Opposite effect of acute and subchronic treatments with Ferula hermonis on copulatory behavior of male rats [Articolo su rivista]
Zanoli, P.; Benelli, A.; Rivasi, M.; Baraldi, C.; Vezzalini, F.; Baraldi, M.
abstract

Sexually potent and sluggish/impotent male rats were orally treated with an extract of Ferula hermonis (30 and 60 mg/kg). The acute administration stimulated sexual motivation in potent rats and improved copulatory performance in sluggish/impotent rats. This last effect was elicited only by the higher dose, which, in parallel, increased serum testosterone levels in rats. On the contrary, when the extract was subchronically administered (10 days) a marked reduction in the percentage of rats achieving ejaculation was detected, together with a general impairment of the copulatory pattern. Furthermore, the repeated administration of the extract (6 mg/kg/day for 10 days) resulted in a significant reduction of testosterone levels in comparison with controls. The present results discourage a repeated assumption of F. hermonis, while suggesting its acute administration to improve the performance in sexual dysfunctions.

2002 - Synthesis and antiplatelet activity of gemfibrozil chiral analogues [Articolo su rivista]
Ammazzalorso, A.; Amoroso, R.; Baraldi, M.; Bettoni, G.; Braghiroli, D.; De Filippis, B.; Duranti, A.; Moretti, M.; Tortorella, P.; Tricca, M. L.; Vezzalini, F.
abstract

The chiral analogues of gemfibrozil 5-(2,5-dimethylphenoxy)-2-methylpentanoic acid and 5-(2,5-dimethylphenoxy)-2-ethylpentanoic acid were synthesized in optically active form using (S)-4-(1-methylethyl)-2-oxazolidinone as chiral auxiliary. All compounds inhibit human platelet aggregation. From these data, one can surmise that all tested compounds and gemfibrozil act at the platelet level with different mechanism than that of ASA, even if with a different potency. © 2002 Elsevier Science Ltd. All rights reserved.

1998 - Pharmacological profile of flavonoids present in medicinal plants with sedative and axiolytic properties [Abstract in Atti di Convegno]
Zanoli, Paola; Avallone, Rossella; Truzzi, Cristina; Vezzalini, Francesca; Baraldi, Mario
abstract

In the present study we investigated the pharmacological effect of two flavonoids, chrysin and apigenin, contained in Passiflora incarnata and Matricaria chamomilla respetively.

1996 - Simultaneous separation and identification of hashish constituents by coupled liquid chromatography mass spectrometry (HPLC-MS) [Articolo su rivista]
Rustichelli, Cecilia; Ferioli, Valeria; Vezzalini, Francesca; Rossi, M. C.; Gamberini, G.
abstract

This paper reports the use of liquid chromatography for the separation and determination of the major cannabinoids extracted from hashish samples. The direct coupling to the mass spectrometer enables the selective identification both of neutral and acidic cannabinoids. The developed method does not require any preliminary derivatization and should, therefore, be of interest in forensic analysis for simple and unequivocal determination of hashish constituents.

1995 - ANALYSIS OF PYRITHIONES BY REVERSED-PHASE HIGH-PERFORMANCE LIQUID-CHROMATOGRAPHY [Articolo su rivista]
Ferioli, Valeria; Rustichelli, Cecilia; Vezzalini, Francesca; Gamberini, G.
abstract

An analytical procedure has been developed for the determination of zinc pyrithione in antidandruff formulations. Zinc pyrithione was converted into a stable copper complex and then analysed by reversed-phase HPLC. The proposed method allows the separation of the analyte from related pyrithiones and therefore is able to verify the compliance of cosmetic preparations with current legislation.

1995 - Determination of Zinc Pyrithione and related compounds in hair care formulations by HPLC [Abstract in Atti di Convegno]
Ferioli, Valeria; Rustichelli, Cecilia; Vezzalini, Francesca; Gamberini, Gianfranco; G., Lugli
abstract

An analytical procedure has been developed for the determination of Zinc Pirithione in antidandruff formulations. The analyte was converted into a stable copper complex and then analysed by RP-HPLC. Moreover the proposed method allows the separation of zinc pirithione from the related pirithiones

1995 - HIGH-PERFORMANCE LIQUID-CHROMATOGRAPHY OF DIHYDROXYACETONE AS ITS BIS-2,4-DINITROPHENYLHYDRAZONE DERIVATIVE [Articolo su rivista]
Ferioli, Valeria; Vezzalini, Francesca; Rustichelli, Cecilia; Gamberini, G.
abstract

A rapid and simple HPLC method for the identification and determination of dihydroxyacetone, a tanning agent widely used in a variety of preparations, is presented. The method consists of the analysis of the formulated products after a derivatization reaction which enhances the response and the detection limit of the chromatographic analysis. The characterization and the elucidation of the spacial geometry of the synthesized derivative are described.

1995 - HPLC analysis and structural characterisation of dihydroxyacetone as its bis-2,4-dinitrophenylhydrazone derivative [Abstract in Atti di Convegno]
Ferioli, Valeria; Vezzalini, Francesca; Rustichelli, Cecilia; Gamberini, Gianfranco; G., Lugli
abstract

A rapid and simple HPLC procedure for the determination of dihydroxyacetone has been performed; the method involves the analysis of the examined formulations after a derivatisation reaction with 2,4-dinitrophenylhydrazine in order to enhance the detection limit.

1995 - Investigations of Cannabis constituents by HPLC/MS [Abstract in Atti di Convegno]
Rustichelli, Cecilia; Vezzalini, Francesca; Rossi, Maria Cecilia; Ferioli, Valeria; G., Lugli; Gamberini, Gianfranco
abstract

An analytical HPLC/MS procedure was developed for the analysis of major neutral and acidic Cannabis constituens without the need for a derivatisation step of any kind

1994 - CALORIMETRIC AND INFRARED SPECTROPHOTOMETRIC STUDY OF ETHAMBUTOL DIHYDROCHLORIDE [Articolo su rivista]
Gamberini, Gianfranco; Ferioli, Valeria; Rustichelli, Cecilia; Vezzalini, Francesca
abstract

The binary phase diagram of [S,S(+)]-ethambutol dihydrochloride, a chemotherapeutic drug, and its [R,R(-)]-enantiomer, has been determined by IR spectroscopy, X-ray podwer diffraction and differential scanning calorimetry, the equimolecular mixture of [S,S(+)]/[R,R(-)] corresponds to a racemic compound. The infrared spectra of enantiomers and racemic compound differ especially in the finger print region. The solid-solid transition and fusion enthalpies and entropies for the optically active compounds and the corresponding racemic compound have been determined. The stability of the racemic compound has been evaluated on the basis of free energy of formation. In this paper the results of calorimetric and spectroscopic analyses for the determination of enantiomeric purity of ethambutol dihydrochloride have been reported.

1994 - DETERMINATION OF AZELAIC ACID IN PHARMACEUTICALS AND COSMETICS BY RP-HPLC AFTER PRECOLUMN DERIVATIZATION [Articolo su rivista]
Ferioli, Valeria; Rustichelli, Cecilia; Vezzalini, Francesca; Gamberini, Gianfranco
abstract

This paper reports a RP-HPLC method for the determination in topics of azelaic acid, a keratolytic and anti-comedogenic agent widely used in the treatment of all types of acne. A derivatization step was needed prior to chromatographic analysis because the analyte is lacking in chromophore. A sample clean-up procedure by solid-phase extraction was also developed to analyse azelaic acid in complex matrices, such as pharmaceutical and cosmetic formulations.

1994 - DIRECT DETERMINATION OF NON-UV-ABSORBING COMPOUNDS BY HIGH-PERFORMANCE LIQUID-CHROMATOGRAPHY [Articolo su rivista]
Ferioli, Valeria; Gamberini, Gianfranco; Rustichelli, Cecilia; Vezzalini, Francesca
abstract

A direct liquid chromatographic method with UV detection has been developed for the determination of ethambutol dihydrochloride, a chemotherapeutic drug lacking in chromophoric groups. It allows the simultaneous separation of [S,S(+)]-ethambutol from meso-[S,R(+/-)]-form and its synthetic precursor [S(+)]-2-amino-1-butanol, although it is not stereospecific for the separation of the [R,R(-)]-enantiomer. Important factors affecting the selectivity and efficiency of separation (CuSO4 concentration, temperature and addition of an organic modifier in the mobile phase) have been studied. Moreover the proposed method allows the quantitative determination of ethambutol dihydrochloride in pharmaceutical formulations in order to verify the agreement with the claimed content for the drug.

1993 - Calorimetric and infrared spectrophotometric study of (S,S)-2,2?-(1,2ethanediyldiimino)bis-1-butanol hydrochloride [Abstract in Atti di Convegno]
Gamberini, Gianfranco; Ferioli, Valeria; Rustichelli, Cecilia; Vezzalini, Francesca
abstract

This paper reports the results of calorimetric and IR analysis for the characterisation of the solid state and for the determination of the enantiomeric purity of Ethambutol Hydrochloride.

1993 - Determination of medium chain dicarboxylic acids in pharmaceuticals and cosmetics by SPE and RP-HPLC analysis [Abstract in Atti di Convegno]
Ferioli, Valeria; Rustichelli, Cecilia; Vezzalini, Francesca; Gamberini, Gianfranco
abstract

A method has been developed for the extraction and determination of azelaic acid in pharmaceutical and cosmetic formulations. The method includes sample clean-up using SPE extraction, preparation of UV-absorbing derivatives and RP-HPLC separation.

1993 - Direct determination of non-UV absorbing compounds by high-performance liquid chromatography [Abstract in Atti di Convegno]
Ferioli, Valeria; Gamberini, Gianfranco; Rustichelli, Cecilia; Vezzalini, Francesca
abstract

A direct liquid chromatographic method has been developed for the determination of Ethambutol Hydrochloride, allowing the separation of its enantiomeric couple from the meso form. The analyses were performed on a LEC-Chiralsil D-ValCu column and the analytes compounds were detected by UV absorption as their copper chelates.

1992 - DETERMINATION OF BRONIDOX AND BRONOPOL IN COSMETIC PRODUCTS BY REVERSED PHASE HIGH-PERFORMANCE LIQUID-CHROMATOGRAPHY [Articolo su rivista]
Ferioli, Valeria; Vezzalini, Francesca; Rustichelli, Cecilia; Gamberini, G.
abstract

A rapid and simple HPLC method is described for the separation and quantitative determination of bronidox and bronopol used as preservatives and listed in the current EEC Council Directives on cosmetic products. The proposed method allows the routine detection of these preservatives in different cosmetic formulations with a view for verifying their complicance with Italian legislation.

1991 - Analysis of bronidox and Bronopol in cosmetic products by reversed phase high-performance liquid chromatography [Abstract in Atti di Convegno]
Ferioli, Valeria; Vezzalini, Francesca; Rustichelli, Cecilia; Gamberini, Gianfranco
abstract

This paper reports a rapid and simple HPLC method for the separation and quantitative determination of bronidox and bronopol used as preservatives; the developed method allows to verify the compliance of commercial products with current European Economic Community (EEC) Council Directive.

1990 - Separation of chiral compounds with alpha-acid glycoprotein as selector [Articolo su rivista]
Gamberini, Gianfranco; Ferioli, Valeria; Rustichelli, Cecilia; Vezzalini, Francesca
abstract

The present paper reports an optimized procedure for the efficient and fast HPLC-CSP separation of the enantiomeric pair of verapamil uusing a chiral column with alpha-acid glycoprotein as selector. The influence of temperature, pH, flow-rate and concentration of organic modifier on the chromatographic separations is shown.

1990 - Separation of chiral compounds with alpha-acid glycoprotein as selector [Abstract in Atti di Convegno]
Gamberini, Gianfranco; Ferioli, Valeria; Rustichelli, Cecilia; Vezzalini, Francesca
abstract

The present paper reports an optimized procedure for the efficient and fast HPLC separation of the enantiomeric pair of verapamil hydrochloride using an AGP chiral stationary phase. The influence of pH, flow-rate, temperature and concentration of organic modifier was investigated.