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MARIANNA RIVASI

Docente a contratto
Dipartimento Chirurgico, Medico, Odontoiatrico e di Scienze Morfologiche con interesse Trapiantologico, Oncologico e di Medicina Rigenerativa

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Pubblicazioni

2021 - Adherence to Lipid-Lowering Medication in People Living with HIV: An Outpatient Clinic Drug Direct Distribution Experience [Articolo su rivista]
Cuomo, Gianluca; Raimondi, Alessandro; Rivasi, Marianna; Guaraldi, Giovanni; Borghi, Vanni; Mussini, Cristina
abstract

Adherence to lipid-lowering drugs could be challenging in our patients as it is in the general population, which is described as low as 25%. Our aim was to evaluate adherence to statins and to investigate clinical event impact on it.

2011 - Experimental study on Satureja montana as a treatment for premature ejaculation [Articolo su rivista]
Zavatti, Manuela; Zanoli, Paola; Benelli, Augusta; Rivasi, Marianna; Baraldi, Claudia; Baraldi, Mario
abstract

AbstractAim of the studySatureja montana (winter savory) is a medicinal plant traditionally used to treat different disorders including male sexual dysfunction. In this study we evaluated the effect of Satureja montana hydroalcoholic extract on copulatory behavior of sexually potent male rats.Materials and methodsThe extract was orally administered acutely or repetitively for 8 consecutive days at the doses of 25 and 50 mg/kg. The main parameters of sexual behavior, mount (ML), intromission (IL), ejaculation (EL) latencies and post-ejaculatory interval (PEI), were evaluated in animals submitted to mating test and multiple ejaculations test. Testosterone serum levels were measured in rats acutely treated with Satureja montana extract dosed at 50 mg/kg. In addition the open field test was conducted to evaluate the locomotor behavior.ResultsWhen acutely administered at both dosages, the extract was able to significantly increase EL and decrease intromission frequency (IF) in comparison with controls. The significant increase in EL was found also when the extract was subacutely administered, daily for 8 consecutive days, at the dose of 25 mg/kg. In the multiple ejaculations test, EL values of treated rats were significantly increased during the 1st and 2nd sequence in comparison with controls; in addition only rats treated with the extract were able to reach the 4th ejaculation within 30 min. Testosterone serum level measured in rats acutely treated with Satureja montana at the dose of 50 mg/kg was significantly increased in rats in comparison with controls. Finally, the locomotor activity recorded in the open field test was not affected by the acute administration of the plant extract.ConclusionsThese data suggest that Satureja montana could be considered as a natural remedy for the treatment of premature ejaculation delaying ejaculation latency without exerting any negative effect on the other parameters of sexual behavior and without exerting a sedative effect. In addition the increased serum level of testosterone confirms the positive influence of Satureja montana on male sexual function.

2009 - Experimental evidence of the anaphrodisiac activity of Humulus lupulus L. in naïve male rats [Articolo su rivista]
Zanoli, Paola; Zavatti, Manuela; Rivasi, Marianna; Benelli, Augusta; Avallone, Rossella; Baraldi, Mario
abstract

Ethnopharmacological relevance: In the folk medicine Humulus lupulus L. (hops) is mainly recommendedas a mild sedative with antispasmodic and digestive properties. It is also reputed to exert an anaphrodisiaceffect but it is still lacking the experimental evidence of this activity.Aim of the study: To evaluate the influence of Humulus lupulus extract on sexual behavior of both naïveand sexually potent male rats; thereafter to investigate the role of 8-prenylnarigenin (8-PN) in the effectdisplayed by the hop extract.Materials and methods: Sprague–Dawley male rats both naïve and sexually potent were acutely administeredwith the hop extract dosed at 5, 10, 25 and 50 mg/kg. In addition the extractwas administered dailyfor 10 consecutive days at the dose of 0.25 mg/kg/day in sexually potent animals. The pure compound8-PN was acutely administered in naïve rats at the dosages of 5, 12.5 and 25g/kg. All the animals werescreened for their sexual behavior manifestation during the mating test.Results: In naïve rats the acute administration of Humulus lupulus extract at the doses of 25 and 50 mg/kgsignificantly reduced the percentage of mounting and ejaculating animals, in comparison to vehiclecontrols. The other parameters recorded during the mating test were not affected by the hop extract.In sexually potent rats nor the acute neither the repeated administration of the extract modified theircopulatory behavior.The pure compound 8-PN failed to influence male sexual behavior of naïve rats.Conclusion: Humulus lupulus extract exerted an anaphrodisiac effect only in naïve rats by inhibiting theirmounting and ejaculating behavior. The presence of 8-PN in the extract could be only partially involvedin the observed anaphrodisiac effect.

2008 - Improved sexual behavior in male rats treated with a Chinese herbal extract: hormonal and neuronal implications [Articolo su rivista]
Zanoli, Paola; Benelli, Augusta; Zavatti, Manuela; Rivasi, Marianna; Baraldi, Claudia; Baraldi, Mario
abstract

Aim: To investigate the influence of an extract obtained from five Chinese medicinal plants on sexual behavior ofadult male rats. Methods: The extract was administered at doses of 30, 60 and 120 mg/kg by oral gavage, acutely (onetime, 45 min before mating test) or subchronically (daily for 10 days) in sexually potent and sexually sluggish/impotentrats. Sexual behavior, serum levels of luteinizing hormone (LH) and testosterone (T) were evaluated in treatedrats and compared with controls receiving vehicle. The effect of the extract on central dopaminergic neurotransmissionwas assessed in the nucleus accumbens using a microdialysis technique. Results: In sexually potent rats, bothacute and subchronic treatment with the extract dosed at 30 and 60 mg/kg reduced mount latency and intromissionlatency. In sluggish/impotent rats, the acutely administered extract at the dose of 60 mg/kg shortened ejaculationlatency, whereas subchronically administered at the doses of 30 and 60 mg/kg, reduced mount, intromission andejaculation latencies, increasing also the percentage of mounting and ejaculating rats. The extract dosed at 60 mg/kgsignificantly increased LH and T following acute and subchronic administration and increased 3,4-dihydroxyphenylaceticacid levels in the nucleus accumbens, 30 min after the acute administration. Conclusion: The improvement in bothappetitive and consummatory components of sexual behavior observed in male rats treated with the extract could beascribed to increased serum T level in parallel with the activation of the central dopaminergic system.

2007 - Evidence that the β-acid fraction of hops reduces central GABAergic neurotransmission [Articolo su rivista]
Zanoli, Paola; Zavatti, Manuela; Rivasi, Marianna; F., Brusiani; Losi, Gabriele; Puja, Giulia; Avallone, Rossella; Baraldi, Mario
abstract

Humulus lupulus (hops) is traditionally used as a tranquilizing herbal remedy. Here, we investigated the in vivo and in vitro effect of hop β-acids on central nervous system function. Oral administration of β-acids (5–10 mg/kg) in rats produced an increased exploratory activity in the open field, a reduction in the pentobarbital hypnotic activity and a worsening of picrotoxin-induced seizures. When dosed at 10 mg/kg, β-acids increased, in the elevated plus maze, open arm entries reducing in parallel those in closed arms. In the forced swimming test, we observed a reduction in the immobility time that could suggest an antidepressant-like activity. Electrophysiological studies performed on cerebellar granule cells in culture showed that the β-acids fraction decreased GABA-evoked current in a dose-dependent way. The effect was not inhibited by the benzodiazepine antagonist Ro 15-1788. Benzodiazepine receptors involvement was also excluded by [3H]-Ro 15-1788 binding assay. In conclusion, the behavioral effects of β-acids fraction could be explained by a reduction in the GABAergic activity although we cannot rule out the involvement of other neurotransmitter systems

2007 - SOPRAVVIVENZA E CRESCITA DI DIVERSI SIEROGRUPPI DI LEGIONELLA PNEUMOPHILA NELL’AMBIENTE ACQUATICO [Abstract in Rivista]
Marchesi, Isabella; Bargellini, Annalisa; Cauteruccio, Laura; Rivasi, Marianna; Guerrieri, Elisa; Messi, Patrizia; Borella, Paola
abstract

Obiettivo. Scopo dello studio era di verificare la capacità di sopravvivenza e crescita, in forma libera e nel biofilm, di ceppi di Legionella pneumophila appartenenti a diversi sierogruppi (sgs) in un sistema sperimentale che simula un impianto distribuzione di acqua calda.Materiali e metodi. Il modello da noi allestito è paragonabile a quello di un condominio di medie dimensioni, dove l’acqua a 40°C circola mediante una pompa. Dopo la stabilizzazione del sistema, sono stati inoculati 107 ufc/L ciascuno dei sgs 1, 6 e 9. I prelievi dell’acqua e del biofilm formato su un tubo posto all’interno della vasca sono stati effettuati settimanalmente per 3 mesi.Risultati. Dopo una settimana dall’inoculo, si è osservata una netta prevalenza del sg 6, pari al 70% sugli altri due ceppi. Tale trend si è confermato nel tempo e dopo sette settimane il sg 6 rappresentava il 93% degli isolati, contro un 7% del sg 1 e un valore prossimo allo 0% per il sg 9. Successivamente, l’andamento si è modificato, con un progressivo aumento del sg 1 che a 90 giorni rappresentava il 47% degli isolati contro il 41% del sg 6 e il 12% del sg 9. Andamenti del tutto analoghi sono stati osservati anche nei campioni di biofilm.Conclusioni. I risultati confermano che a parità di inoculo, ceppi appartenenti a diversi sierogruppi della specie L. pneumophila si diversificano sia nella formazione del biofilm che nella esistenza in forma planktonica. Tra i ceppi saggiati, l’adattabilità maggiore era del sg 6, seguita dai sgs 1 e 9. Va inoltre osservato che la percentuale del sg 1 (dal 7% al 47% delle legionelle isolate) sembra rispecchiare quella ritrovata in condizioni reali, sia nelle abitazioni che negli alberghi, a conferma della validità del nostro modello sperimentale per indagare sui meccanismi di crescita ambientale di Legionella spp.

2005 - Activity of single components of Ferula hermonis on male rat sexual behavior [Articolo su rivista]
Zanoli, Paola; Rivasi, Marianna; Zavatti, Manuela; F., Brusiani; Vezzalini, Francesca; Baraldi, Mario
abstract

The influence of the single components of Ferula hermonis extract on sexual behavior was studied in male rats. Sexually potent and sluggish/impotent animals were orally treated acutely (2.5 mg/kg) and subchronically (0.25 mg/kg/day for 10 days) with ferutinin, teferdin and teferin. Ferutinin alone acutely administered in potent rats was able to reduce mount and intromission latencies, while in sluggish/impotent animals, it induced the same effects and additionally shortened the ejaculation latency, as teferdin did. Both substances increased testosterone levels in rats. Unlike teferdin, ferutinin subchronically administered in potent rats negatively affected appetitive and consummatory sexual behavior, reducing also testosterone serum levels. In conclusion, if repetitively administered, ferutinin was able to stimulate sexual behavior after acute ingestion, but exerted a negative influence on the sexual capacity of potent male rats, whereas teferdin only improved copulatory performance of sluggish/impotent animals.

2005 - Ferula hermonis impairs sexual behavior in hormone-primed female rats [Articolo su rivista]
Zanoli, Paola; Zavatti, Manuela; Rivasi, Marianna; Baraldi, Mario
abstract

The influence of Ferula hermonis root extract on sexual behavior was studied in female rats. Sexual receptivity, proceptivity and paced mating behavior were evaluated in ovariectomized females primed with estradiol benzoate (EB) and progesterone (P) and then treated with F hermonis extract acutely (30 and 60 mg/kg) or subehronically (1 and 10 mg/kg daily for 10 consecutive days). A significant reduction in lordosis responses was observed in rats after acute (60 mg/kg) or subehronic (1 and 10 mg/kg) administration of the plant extract. Similarly, a decrease in proceptive behaviors was exhibited by F hermonis treated rats in comparison with EB + P controls. No difference was found in the patterns of paced mating behavior between control and treated animals. The present results demonstrate that the acute or repeated ingestion of F hermonis specifically impairs the receptive and proceptive components of female sexual behavior. The effect could be the consequence of an antiestrogenic action of the extract in hormone-primed female rats.

2005 - New insight in the neuropharmacological activity of Humulus lupulus L [Articolo su rivista]
Zanoli, Paola; Rivasi, Marianna; Zavatti, Manuela; Brusiani, F.; Baraldi, Mario
abstract

The purpose of the present study was to investigate the effects of Humulus lupulus CO2 extract and its fraction containing α-acids on the central nervous system of rats. Both tested substances were able to prolong pentobarbital sleeping time, without affecting the latency to the loss of the righting reflex. This effect was dose-dependent, starting from a minimal dose of 10 mg/kg. Neither the extract nor its α-acid fraction affected the locomotor activity in the open field test or exerted an anxiolytic effect in rats submitted to the elevated plus-maze test. Interestingly both compounds reduced the immobility time during the behavioral despair test when administered three times (24, 5 and 1 h) before the test. In conclusion this report shows that Humulus lupulus CO2 extract exerts: (a) a pentobarbital sleep-enhancing property without influencing the motor behavior of rats; (b) an antidepressant activity. The same effects were elicited by the administration of the Humulus lupulus fraction containing α-acids, which can be considered as the major responsible for the enhanced pentobarbital effect and for the antidepressant property.

2005 - Pharmacological characterisation of beta-acids fraction of hops [Abstract in Atti di Convegno]
Zavatti, Manuela; Zanoli, Paola; Rivasi, Marianna; Avallone, Rossella; Baraldi, Mario
abstract

Humulus lupulus (hops) is traditionally used as a tranquilizing herbal remedy. Here, we investigated the in vivo and in vitro effect of hop -acids on central nervous system function. Oral administration of -acids (5–10 mg/kg) in rats produced an increased exploratory activity in the open field, a reduction in the pentobarbital hypnotic activity and a worsening of picrotoxin-induced seizures. When dosed at 10 mg/kg, -acids increased, in the elevated plus maze, open arm entries reducing in parallel those in closed arms. In the forced swimming test, we observed a reduction in the immobility time that could suggest an antidepressant-like activity. Electrophysiological studies performed on cerebellar granule cells in culture showed that the -acids fraction decreased GABA-evoked current in a dose-dependent way. The effect was not inhibited by the benzodiazepine antagonist Ro 15-1788. Benzodiazepine receptors involvement was also excluded by [3H]-Ro 15-1788 binding assay. In conclusion, the behavioraleffects of -acids fraction could be explained by a reduction in the GABAergic activity although we cannot rule out the involvement of other neurotransmitter systems.

2001 - Sedative and anxiolytic properties od chrysin, a constituent of Passiflora incarnata [Abstract in Atti di Convegno]
Rivasi, Marianna; Muscatello, Cinzia; Zanoli, Paola; Avallone, Rossella; Baraldi, Mario
abstract

The behavioral effects of acute administration of chrysin, contained in Passiflora incarnata, were studied in rats. The data demonstrate that in our experimental conditions, chrysin was able to reduce locomotor activity when injected in rats at a minimal effective dose of 25 mgrkg. However, chrysin exhibited a clear anxiolytic effect when injected at the dose of 1 mgrkg,. The sedative effect of the flavonoid cannot be ascribed to an interaction with GABA]benzodiazepine receptors, since it was not counteracted by the benzodiazepine antagonist Flumazenil. To the contrary, the anxiolytic effect of chrysin, which was blocked by the injection of Flumazenil, could be linked to an activation of the GABAA receptor unit.

2000 - Caratterizzazione dell'attività farmacologica dell'iperforina in confronto all'estratto di Hypericum perforatum [Abstract in Atti di Convegno]
Muscatello, Cinzia; Baraldi, Claudia; Rivasi, Marianna; Avallone, Rossella; Zanoli, Paola
abstract

Allo scopo di chiarire il meccanismo con cui l'iperforina svolge la sua azione ansiolitica, sono stati eseguiti studi preliminari con antagonisti famcaologici che hanno suggerito un possibile coinvolgimento del sistema serotoninergico e una scarsa implicazione del sistema recettoriale benzodiazepinico.

2000 - Characterisation of pharmacoligical activity of hyperforin in comparison with Hypericum extract [Abstract in Atti di Convegno]
Zanoli, Paola; Muscatello, Cinzia; Baraldi, Claudia; Rivasi, Marianna; Avallone, Rossella
abstract

In our experiments we studied the influence of hyperforin on locomotor behavior of rats and evaluated its effect in two experimental models of anxiety in comparison with the administration of hypericum extract.

2000 - Definizione delle attività farmacologiche dell'iperforina e relativi meccanismi d'azione [Abstract in Atti di Convegno]
Zanoli, Paola; Muscatello, Cinzia; Baraldi, Claudia; Rivasi, Marianna; Avallone, Rossella
abstract

nel nostro studio abbiamo preso in considerazione l'effetto dell'iperforina sul comportamento motorio del ratto e la sua influenza in modelli sperimentali di ansia. In tali esprimenti abbiamo messo a confronto l'attività dell'iperforina acetato con quella di un estratto totale di iperico ottenuto con la tecnica della CO2 supercritica.