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Centro Interdipartimentale Cellule Staminali e Medicina Rigenerativa (CIDSTEM)
Centro Interdipartimentale Cellule Staminali e Medicina Rigenerativa (CIDSTEM)

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2022 - The cell as a tool to understand and repair urethra [Capitolo/Saggio]
Sceberras, Virginia; Maria Magrelli, Federica; Adamo, Davide; Maurizi, Eleonora; Attico, Eustachio; Giuseppe Genna, Vincenzo; Lazzeri, Massimo; Barbagli, Guido; Pellegrini, Graziella

2021 - Regenerative Medicine of Epithelia: Lessons From the Past and Future Goals [Articolo su rivista]
Maurizi, Eleonora; Adamo, Davide; Magrelli, FEDERICA MARIA; Galaverni, Giulia; Attico, Eustachio; Merra, Alessia; Maffezzoni, MARIA BENEDETTA RIZZARDA; Losi, Lorena; Genna, VINCENZO GIUSEPPE; Sceberras, Virginia; Pellegrini, Graziella

This article explores examples of successful and unsuccessful regenerative medicine on human epithelia. To evaluate the applications of the first regenerated tissues, the analysis of the past successes and failures addresses some pending issues and lay the groundwork for developing new therapies. Research should still be encouraged to fill the gap between pathologies, clinical applications and what regenerative medicine can attain with current knowledge.

2020 - A fine-tuned β-catenin regulation during proliferation of corneal endothelial cells revealed using proteomics analysis [Articolo su rivista]
Maurizi, E.; Schiroli, D.; Zini, R.; Limongelli, A.; Misto, R.; Macaluso, C.; Pellegrini, G.

Corneal endothelial (CE) dysfunction is the main indication for corneal transplantation, an invasive procedure with several limitations. Developing novel strategies to re-activate CE regenerative capacity is, therefore, of fundamental importance. This goal has proved to be challenging as corneal endothelial cells (CEnC) are blocked in the G0/G1 phase of the cell cycle in vivo and, albeit retaining proliferative capacity in vitro, this is further hindered by endothelial-to-mesenchymal transition. Herein we investigated the mechanisms regulating CEnC proliferation in vitro. Comparing the proteome of non-proliferating (in vivo—G0/G1) and proliferating (in vitro—G2/M) rabbit CEnC (rCEnC), 77 proteins, out of 3,328 identified, were differentially expressed in the two groups (p < 0.005). Literature and Gene Ontology analysis revealed β-catenin and transforming growth factor (TGF-β) pathways to be correlated with the identified proteins. Treatment of rCEnC with a β-catenin activator and inhibitor showed that β-catenin activation was necessary during rCEnC proliferation, but not sufficient for its induction. Furthermore, both pro-proliferative activity of basic fibroblast growth factor and anti-proliferative effects of TGF-β were regulated through β-catenin. Overall, these results provide novel insights into the molecular basis underlying the proliferation process that CEnC re-activate in vitro, consolidating the role of β-catenin and TGF-β.