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VINCENZA RITA LO VASCO

Professore Associato
Dipartimento di Scienze Biomediche, Metaboliche e Neuroscienze,sede Istituti Anatomici (area Policlinico)

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Pubblicazioni

2024 - Specific subcellular localization of phosphoinositide-specific phospholipase C enzymes in different human osteosarcoma cell lines [Articolo su rivista]
Corradini, M; Checchi, M; Benincasa, M; Ferretti, M; Cavani, F; Palumbo, C; LO VASCO, VINCENZA RITA
abstract

The role of signal transduction in cancer progression is well established and actively studied, including in osteosarcoma. The signal transduction pathways involved in the regulation of calcium metabolism are being intensively studied, with particular regard to phosphoinositide-specific phospholipase C (PLC) signaling. This family of enzymes helps to modulate calcium metabolism and is interconnected with additional signaling molecules belonging to different pathways. The expression and subcellular localization of PLCs have been shown to differ in normal cells compared to their neoplastic counterpart in different types of cancer. We now describe the localization of the PLC enzyme family in 4 human osteosarcoma cells different in origin and malignancy (MG63, U2OS, HOS and 143B cell lines). We identified cell line-specific differences and discussed possible meaning and implications.

2023 - Emerging Roles of Signal Transduction Pathways in Neurodegenerative Diseases. Hunting New Possible Therapeutic Molecular Targets [Articolo su rivista]
LO VASCO, VINCENZA RITA
abstract

Illnesses following the degeneration of the nervous system can occur due to aging or genetic mutations and represent a clinical concern. In neurodegenerative diseases, loss of neuronal structure and functions mainly causes cognitive impairment, representing an increasing social burden. In neurodegenerative diseases, the progressive loss of vulnerable populations of neurons in specific regions of the central nervous system was traced to different pathological events, such as misfolded proteins’ accumulation, abnormalities in proteasomes or phagosomes, as well as anomalies in lysosomes or mitochondria. Many research efforts identified important events involved in neurodegeneration, but the complex pathogenesis of neurodegenerative diseases is far from being fully elucidated. More recently, insights into the signal transduction pathways acting in the nervous system contributed to unveiling some molecular mechanisms triggering neurodegeneration. Abnormalities in the intra- or inter-cellular signaling were described to be involved in the pathogenesis of neurodegenerative disease. Understanding the signal transduction pathways that impact the nervous system homeostasis can offer a wide panel of potential targets for modulating therapeutic approaches. The present review will discuss the main signal transduction pathways involved in neurodegenerative disorders.

2023 - Endoplasmic reticulum localization of phosphoinositide specific phospholipase C enzymes in U73122 cultured human osteoblasts [Articolo su rivista]
Corradini, Matteo; Checchi, Marta; Ferretti, Marzia; Cavani, Francesco; Palumbo, Carla; LO VASCO, VINCENZA RITA
abstract

Different signal transduction pathways contribute to the differentiation and metabolic activities of osteoblasts, with special regard to the calcium-related pathway of phosphoinositide specific phospholipase C (PLC) enzymes family. PLC enzymes were demonstrated to be involved in the differentiation of osteoblasts, and differently localize in the nucleus, cytoplasm or both depending on the isoform. The amino-steroid molecule U-73122 inhibits the enzymes belonging to the PLC family. Beside the temporary block of the enzymatic activity, U-73122 promotes off-target effects, including modulation of the expression of selected PLC genes and different localization of PLC enzymes depending on the cell line in different cell lines. In order to evaluate possible off-target effects of the molecule in human osteoblasts, we investigated the expression of PLC genes and the localization of PLC enzymes in cultured human osteoblasts (hOBs) in the presence of low dose U-73122. Our results confirm that all PLC genes are transcribed in hOBs, that probably splicing variants of selected PLC genes are expressed, and that all PLC enzymes are present in hOBs, excepting for PLC 3 in quiescent hOBs at seeding. Our results confirm literature data excluding toxicity of U-73122 upon cell survival. Our results indicate that U-73122 did not significantly affect the transcription of PLC genes. It acts upon the localization of PLC enzymes, as PLC enzymes are detected in cell protrusions or pseudopodia-like structures, at the nuclear or the plasma membrane, in membrane ruffles, and/or in the endoplasmic reticulum.

2023 - The role of Phosphoinositide-specific Phospholipase C enzymes in normal, regenerating and pathological liver [Capitolo/Saggio]
LO VASCO, VINCENZA RITA
abstract

The liver acts crucially in different physiological processes, including storage and metabolism of nutrients, synthesis of molecules and purification of chemicals, thus representing a complex metabolic system. A plethora of factors, including toxic chemicals, viruses, drugs, and alcohol can disrupt the normal functions leading to different liver illnesses, such as steatosis, hepatitis, fibrosis/cirrhosis, and cancer. Numerous different signal transduction pathways contribute to the organization of the complex activities of the liver. The phosphoinositide (PI) signal transduction pathway was described to participate in liver metabolism and abnormal activity or expression of PI‐specific phospholipase C (PLC) enzymes was described in liver diseases. PLC enzymes are functionally connected to a number of regulatory molecules, including G protein subunits, small GTPases from Rho and RAS families, receptor and non‐receptor tyrosine kinases, and further lipid components of the cell plasma-membrane. In liver, selected PLC isoforms interact and are abnormally expressed in a conspicuous number of human liver anomalies, i.e. in the progression of hepatocellular carcinoma (HCC). In human hepatic angiosarcoma, PLC enzymes were demonstrated to contribute resistance to chemotherapy and PLCs were involved in mouse hepatoma ascites in high metastatic potential cell lines. PLC enzymes were also claimed to play a role in the histopathology features observed in the liver following alcohol abuse. In this review the involvement of the activity of PLC enzymes in normal, regenerating and pathological liver will be described.

2023 - Unexpected Absence of Skeletal Responses to Dietary Magnesium Depletion: Basis for Future Perspectives? [Articolo su rivista]
Ferretti, Marzia; Cavani, Francesco; Lo Vasco, Vincenza Rita; Checchi, Marta; Truocchio, Serena; Davalli, Pierpaola; Frassineti, Chiara; Rizzi, Federica; Palumbo, Carla
abstract

2022 - Expression and localization of Phosphoinositide-specific Phospholipases C in cultured, differentiating and stimulated human osteoblasts [Articolo su rivista]
Casoni Sara, Daisy; Romanelli, Alessia; Checchi, Marta; Truocchio, Serena; Ferretti, Marzia; Palumbo, Carla; LO VASCO, VINCENZA RITA
abstract

The osteoblasts contribute to bone homeostasis maintaining the bone mass, and intervene in bone injuries repair. The limited number of available therapeutic agents promoting osteogenesis aroused the greatest interest in the control of osteoblasts’ activity. Insights in the events leading to the proliferation and differentiation of osteoblasts might allow uncover potential molecular targets to control the complex mechanisms underlying bone remodeling. Oscillations of calcium act crucially during this remodeling, affecting both the differentiation and proliferation of osteoblasts. Signal transduction pathways contribute to the differentiation and metabolic activities of osteoblasts, with special regard to calcium-related signaling, including the Phosphoinositide (PI) pathway and related Phospholipases C (PLCs). In order to evaluate the role of PLC enzymes’ family in human osteoblasts (HOBs), we analyzed the expression of PLC genes and the localization of PLC enzymes in cultured HOBs and in in vitro differentiating HOBs after 3, 10, 17 and 23 days, and in HOBs stimulated with Lipopolysaccharide, which affects the differentiation of osteoblasts, after 3, 6, 24 and 48 hours. Our results confirm the transcription of most PLC genes and the presence of a number of PLC enzymes in HOBs, differently localized in the nucleus, in the cytoplasm or both, as well as in cell protrusions. The localization of PLC enzymes within the cell suggests the activation of both the PI nuclear and of the cytoplasmic cycle in HOBs. Depending on the experimental conditions, transcripts of splicing variants of selected PLC genes were detected and the localization of most PLC enzymes varied, with special regard to enzymes belonging to the PLC ,  and  sub-families. Further studies addressed to elucidate the complex network involving the signal transduction of PLCs might provide further insights into the complex signal transduction network in bone remodeling, also offering the opportunity to identify promising molecular targets.

2022 - Phosphoinositide-specific Phospholipases C in psychiatric diseases and suicide [Articolo su rivista]
LO VASCO, VINCENZA RITA
abstract

Mood disorders represent a major medical need requiring chronic treatment. About one million people die by suicide worldwide each year, both as a consequence of major depression or not. Multiple deficits, including cell atrophy and loss, were described in the brains of mood disorders affected patients and in experimental animal models. Numerous changes in gene expression and activity were described in limbic and cortical brain regions. Available therapies probably regulate many of these changes. Different signal transduction pathways play a role in the pathogenesis of schizoaffective disorders, namely the cyclic‐AMP, phosphoinositides (PI), mitogen‐activated protein kinase, and glycogen synthase kinase cascades. Neurobiology studies focused upon abnormalities of signalling mechanisms with special regard to the serotonin system and related PI signalling system. Involvement of PI-specific Phospholipase C (PLC) enzymes was also described. In suicide brains the overall PLC expression was altered due to a complex reorganization of the isoforms, and PLC 1 isoform was suggested to be involved in schizophrenia and bipolar disorder. The knowledge of the complex network of neurobiological molecules and interconnected signal transduction pathways in the brain might help to understand the natural history and the pathogenesis of mood disorders, as well as of the suicidal behaviour. Moreover, it might widen the panel of available therapeutic tools, also gaining prognostic suggestions in order to prevent suicide.

2021 - Expression and localization of Phosphoinositide-specific Phospholipases C in cultured and differentiating human osteoblasts [Relazione in Atti di Convegno]
Lo Vasco, V; Casoni, S; Romanelli, Alessia; Checchi, Marta; Palumbo, Carla
abstract

Homeostasis in the bone tissue primarily depends on the balance of the activities of osteoclasts and osteoblasts, primarily involved in bone formation and turnover (Zaidi 2007; Khosla et al 2005). Osteoblasts maintain the bone mass, and intervene in bone injuries repair. The limited number of therapeutic agents able to promote osteogenesis ingenerated great interest addressed to manipulate the activity of osteoblasts. Insights in the events leading to the differentiation and proliferation of osteoblasts might allow uncover potential molecular therapy targets to control the complex mechanisms underlying the skeletal remodeling (Marie 2015; Kawai et al 2011). Oscillations of calcium act crucially during the remodeling of bone, affecting both the differentiation and proliferation of osteoblasts. Signal transduction pathways contribute to the differentiation and metabolic activities of osteoblasts, with special regard to calcium-related signaling (Kimple et al 2011, Keinan et al 2014), including the Phosphoinositide (PI) pathway and related Phospholipases C (PLCs). In order to evaluate the role of PLC enzymes’ family in human osteoblasts (HOBs), we analyzed the expression of PLC genes and the localization of PLC enzymes both in cultured HOBs and in in vitro differentiating HOBs after 3, 10, 17 and 23 days. Our results confirm the transcription of most PLC genes and the presence of a number of PLC enzymes in HOBs, differently localized in the nucleus, in the cytoplasm or both, as well as in cell protrusions. The presence of PLC enzymes within the HOBs suggests the activation of the PI nuclear cycle in HOBs. Along both the culture and differentiation culture periods, transcripts of splicing variants of selected PLC genes were detected and the localization of most PLC enzymes varied, with special regard to enzymes belonging to the PLC ,  and  sub-families. The behavior of selected PLC enzymes will be discussed more in detail. The presented results overall suggest that PLC signaling might provide further insights into the complex signal transduction network in bone remodeling, also representing promising molecular targets.

2021 - Phosphoinositide signal transduction pathway and osteosarcoma metastases [Articolo su rivista]
LO VASCO, VINCENZA RITA
abstract

Metastasis spreading confers worse prognosis to the clinical outcome in patients affected with osteosarcoma, the most common primary bone tumour in childhood and adolescence. The identification of the molecules involved in spreading might help to understand the mechanisms of tumour dissemination, opening the way to novel therapeutic strategies. The activation of ezrin-radixin-moesin (ERM) family proteins was suggested to occur after interaction with molecules belonging to Phosphoinositide signal transduction pathway. The Phosphatydil inositol (4,5) bisphosphate (PIP2), a crucial molecule in PI pathway, was indicated to be involved in the stabilization of ERM proteins or a more efficient receptor binding. The levels of PIP2 in the pathway represent a critical element for regulation of a number of cell events. PIP2 levels are regulated by means of enzymes, including the PI-specific Phospholipase C family. The reduction of PIP2 levels induces ERM protein dissociation from the membrane. PI-PLC enzymes contribute to regulate this event. The role of PI signal transduction molecules in osteosarcoma metastases will be discussed.

2021 - The anatomy and its variants [Capitolo/Saggio]
Palumbo, Carla; Ferretti, Marzia; LO VASCO, VINCENZA RITA
abstract

Visceral and renal artery aneurysms are rare but life-threatening pathologies. Many techniques have been proposed for their treatment, and both open surgery and endovascular strategies have proven to be safe and effective. Many specialists can be involved in the treatment of these aneurysms, from vascular surgeons to interventional radiologists, and the knowledge of every different possible approach is fundamental to offer the patient the best outcome. In this book, all the aspects of visceral and renal artery aneurysms have been widely discussed, from epidemiology and anatomical variations to surgical approaches and materials. To conclude, the authors reported a large series of case reports from different experts in order to obtain a better vision of the real-world practice on this topic.

2020 - Insights into Brain Signal Transduction can Provide Potential Molecular Targets to Approach and Manage Alzheimer’s Disease [Articolo su rivista]
LO VASCO, VINCENZA RITA
abstract

2020 - Phosphoinositide-specific phospholipase C isoforms are conveyed by osteosarcoma-derived extracellular vesicles [Articolo su rivista]
Urciuoli, Enrica; Leopizzi, Martina; Di Maio, Valeria; Petrini, Stefania; D’Oria, Valentina; Giorda, Ezio; Scarsella, Marco; Della Rocca, Carlo; LO VASCO, VINCENZA RITA; Peruzzi, Barbara
abstract

Cancer cells are able to release high amounts of extracellular vesicles, thereby conditioning the normal cells in the surrounding tissue and/or in distant target organs. In the context of bone cancers, previous studies suggested that osteosarcoma cancer cells produce transforming extracellular vesicles able to induce a tumour-like phenotype in normal recipient cells. Indeed, phosphoinositide-specific phospholipase C (PI-PLC) enzymes are differentially expressed in osteosarcoma cell lines with increasing aggressiveness, thus providing helpful insights to better define their role and functions in this bone tumour. By confocal microscopy analysis, we demonstrated that osteosarcoma-derived extracellular vesicles convey all the assessed PI-PLC isoforms, and that they localize into cell membrane bubble-like structures, resembling extracellular vesicles about to be released, as conveyed and/or membrane protein. Cytofluorimetric analysis confirmed the presence of PI-PLC isoforms in the extracellular vesicles collected from conditioned media of osteosarcoma cells. These findings suggest the feasibility to use circulating extracellular vesicles as biomarkers of osteosarcoma progression and/or the monitoring of this distressing disease.

2020 - Supernatants from human osteosarcoma cultured cell lines induce modifications in growth and differentiation in THP-1 cells and in Phosphoinositide specific Phospholipase C enzymes [Articolo su rivista]
Leopizzi, Martina; Di Maio, Valeria; Della Rocca, Carlo; LO VASCO, VINCENZA RITA
abstract

Introduction: molecular components in the microenvironment could affect cell growth, survival/apoptosis and proliferation. Immune system cells respond to molecules, such as cytokines, chemokines and growth factors, produced by the tumor and released in the surrounding microenvironment. Methods: we evaluated the morphological and functional changes in THP-1 cells cultured in culture medium mixed with the culture supernatant of one of three different osteosarcoma (OS) cell lines, namely 143B, HS888 and MG-63 cells. We analyzed the effect of supernatant from OS cultures upon morphology and growth of THP-1 cells, and expression of Phosphoinositide-specific PLC enzymes (PLCs). Results: in supernatants from each OS cell line we identified the presence of selected ILs, of TNF and GM-CSF. Each OS derived supernatant differently modified the growth rate of THP-1 cells, depending on the OS cell line. OS supernatants in the in vitro microenvironment of cells’ culture greatly modified the panel of expression of PLC enzymes expressed by THP-1 cells. THP-1 cells differently express PLC enzymes, depending on the origin of the supernatant. The differences in PLCs’ expression induced by adding OS supernatants to the cultures of THP-1 cells resulted statistically significant for PLCB1 and PLCG2 genes. Conclusions: OS supernatants induce the differentiation of THP-1 cells into macrophages. THP-1 cells cultured in OS supernatants express different panels of expression of PLC enzymes. The panel of expression of PLC enzymes differs during the differentiation of monocyte/macrophage lineage THP-1 cells.

2019 - Phosphoinositide-specific phospholipase C in normal human liver and in alcohol abuse [Articolo su rivista]
Fais, P.; Leopizzi, M.; Di Maio, V.; Longo, L.; Della Rocca, C.; Tagliaro, F.; Bortolotti, F.; Lo Vasco, V. R.
abstract

The phosphoinositide (PI) signal transduction pathway participates in liver metabolism. Abnormal activity or expression of PI-specific phospholipase C (PLC) enzymes has been described in different liver diseases. We resume the role of the PI metabolism in liver and PLC abnormalities in different liver diseases. Moreover, we present the results of PLC analyses in a normal human liver and an alcohol-damaged liver. PLC enzymes and the expression of the corresponding genes in liver biopsies from individuals deceased for complications of the alcoholic liver disease (ALD) at different stages compared with normal controls (deceased individuals with histologically normal livers without alcohol addiction anamnesis) were analyzed by using immunohistochemistry and molecular biology techniques. The expression panel of PLCs was described in normal and alcohol abuse liver. Our observations suggest that the regulation of PLC expression might be due to posttranscriptional events and that alcohol affects the epigenetic control of PLC expression belonging to PI signaling. We also describe the alternate expression of PLCB1 and PLCH1 genes in liver. Our results corroborate literature data suggesting that PLC enzymes are differently expressed in normal versus pathological liver, playing a role in the histopathogenesis of liver tissue damage. The expression and/or localization of selected PLC isoforms is especially affected in alcohol-related liver tissue histopathology. Our present observations confirm that the modulation of protein synthesis plays a role in the regulation of PLC enzymes. We also suggest that this modulation might act at the transcription level. Further studies are required to investigate related epigenetic mechanisms.

2018 - Oral health status in elderly hospitalized patients with dysphagia: The role of dental hygiene [Articolo su rivista]
Longo, L.; Ralli, M.; Clemente, A.; Lo Vasco, V. R.; Greco, A.
abstract

Poor oral health status in elderly hospitalized patients with dysphagia has been shown to increase risk for complications, including aspiration pneumonia. The aim of this study is to investigate oral health status in elderly hospitalized patients with dysphagia and the role of dental hygienists in maintaining their oral health care and avoiding complications. METHODS: This study was conducted on twenty hospitalized patients above 65 years of age with a definite diagnosis of oropharyngeal dysphagia. Oral health was assessed by oral examination, oral hygiene condition evaluation through plaque control record, periodontal disease evaluation with bleeding on probing, presence of caries, salivary pH parameters, oral health habits. RESULTS: Six patients were assisted by dental hygienists, whereas fourteen were not. All patients had a compromised oral health status, regardless the presence of dental hygienists. Patients without a dental hygiene assistance showed significant higher plaque control record scores and bleeding on Probing percentage. CONCLUSIONS: Elderly hospitalized patients with oropharyngeal dysphagia without dental hygiene assistance presented worse oral health status for periodontal disease and caries, with higher risk of severe complications. Given the higher risk of dysphagia complications associated to poor oral care, oral health care in elderly hospitalized patients is recommended.

2018 - The Crucial Roles of Enzymes Belonging to Signal Transduction Pathways. Old Molecules as New Therapy Molecular Targets. Approaches, Perspectives and Criticisms [Articolo su rivista]
Rita Lo Vasco, Vincenza
abstract

2018 - The phosphoinositide signal transduction pathway in the pathogenesis of Alzheimer’s disease [Articolo su rivista]
Lo Vasco, V. R.
abstract

Background: During aging and in age-associated disorders, such as Alzheimer’s Disease (AD), learning abilities decline. Probably, disturbances in signal transduction in brain cells underlie the cognitive decline. The phosphorylation/dephosphorylation imbalance occurring in degenerating neurons was recently related to abnormal activity of one or more signal transduction pathways. AD is known to be associated with altered neuronal Ca 2+ homeostasis, as Ca 2+ accumulates in affected neurons leading to functional impairment. It is becoming more and more evident the involvement of signal transduction pathways acting upon Ca 2+ metabolism and phosphorylation regulation of proteins. A growing interest raised around the role of signal transduction systems in a number of human diseases including neurodegenerative diseases, with special regard to the systems related to the phosphoinositide (PI) pathway and AD. The PI signal transduction pathway plays a crucial role, being involved in a variety of cell functions, such as hormone secretion, neurotransmitter signal transduction, cell growth, membrane trafficking, ion channel activity, cytoskeleton regulation, cell cycle control, apoptosis, cell and tissue polarity, and contributes to regulate the Ca 2+ levels in the nervous tissue. Conclusion: A number of observations indicated that PI-specific phospholipase C (PLC) enzymes might be involved in the alteration of neurotransmission. To understand the role and the timing of action of the signalling pathways recruited during the brain morphology changes during the AD progression might help to elucidate the aetiopathogenesis of the disease, paving the way to prognosis refinement and/or novel molecular therapeutic strategies.

2017 - Anatomia del Pilates [Traduzione in Volume]
LO VASCO, VINCENZA RITA
abstract

This title includes Pilates as you've never seen it before! With detailed descriptions, step-by-step instruction and stunning full-colour anatomical illustrations, "Pilates Anatomy" takes you inside the exercises and programmes that will tone the body, stabilise the core, improve balance and increase flexibility. Using the original mat work of Joseph Pilates, you'll see how key muscles are used, how variations and minor adjustments can influence effectiveness and how breathing, alignment, posture and movement are all fundamentally linked.

2017 - Different Expression and Localization of Phosphoinositide Specific Phospholipases C in Human Osteoblasts, Osteosarcoma Cell Lines, Ewing Sarcoma and Synovial Sarcoma [Articolo su rivista]
Lo Vasco, Vincenza Rita; Leopizzi, Martina; Scotto d’Abusco, Anna; Rocca, Carlo Della
abstract

2017 - Elementi di Anatomia Umana (Gilroy) [Capitolo/Saggio]
Boccafoschi, Francesca; Borsello, Tiziana; Cinti, Saverio; Frontini, Andrea; Galli, Sergio; LO VASCO, VINCENZA RITA; Marmiroli, Paola; Moscheni, Claudia; Protasoni, Marina.
abstract

La formazione medica affronta un’innovativa riorganizzazione che sfida gli studenti, i docenti e gli editori. I programmi dei primi due anni di Medicina sono diventati sempre più multidisciplinari. Pertanto, si è sviluppato il mercato dei libri di testo stile review. I concetti di Anatomia, di Fisiologia, di Istologia, di Embriologia, di Radiologia e anche di Patologia e Immunologia di base sono spesso esposti agli studenti in un unico corso. Nonostante i corsi integrati generino entusiasmo, una loro sfortunata conseguenza è rappresentata dal fatto che gli studenti hanno a disposizione ancora meno tempo per assumere padronanza delle materie consultando grandi libri di testo che le analizzano singolarmente e che in passato hanno rappresentato gli strumenti di apprendimento standard. Questi libri sono spesso i riferimenti più validi per i professionisti e per i docenti mentre gli studenti attualmente consultano testi concisi che analizzano il contesto clinico e che consentono review e test di autovalutazione immediati. Tali testi concisi, insieme alle guide di dissezione e agli atlanti, rappresentano le risorse fondamentali per gli attuali studenti di Anatomia. La nostra sfida è stata quella di fornire un contenuto adeguato in un formato che sia opportuno per questo nuovo stile di apprendimento. Il nostro obiettivo è stato quello di vincere la sfida sviluppando Anatomy – An essential Textbook. Il capitolo introduttivo fornisce spiegazioni chiare riguardanti la terminologia anatomica, i concetti e i sistemi che saranno particolarmente utili agli studenti del primo anno. Il libro è organizzato in Unità per regione (Dorso, Torace, Addome, Pelvi/Perineo, Arto superiore, Arto inferiore e Testa e Collo). Ogni Unità inizia con un capitolo generale che riassume i concetti regionali importanti, così come le caratteristiche dei componenti degli apparati scheletrico e circolatorio e del sistema nervoso della regione analizzata. I capitoli successivi analizzano l’Anatomia degli organi e l’Anatomia funzionale, spesso mediante sottoregioni chiave. Il libro è caratterizzato da oltre 450 eccellenti immagini e da 95 tavole (tra cui molte contengono tabelle e schemi riguardanti muscoli specifici) tratte dal premiato Atlas of Anatomy della Thieme. Inoltre, il testo analizza più di 165 correlazioni cliniche. Ogni Unità è seguita da Domande di verifica che mettono alla prova le conoscenze di base dell’anatomia di quella regione e la sua applicazione clinica. Sono presenti oltre 400 domande. Ci auguriamo che questa combinazione unica degli approcci regionale e sistemico, presentata mediante spiegazioni preliminari puntuali e sostenuta da tabelle riassuntive, da immagini accuratamente scelte, da correlazioni cliniche e da test di autovalutazione, piacerà agli studenti che viaggiano attraverso il mondo dell’Anatomia.

2017 - LPS, Oleuropein and Blueberry extracts affect the survival, morphology and Phosphoinositide signalling in stimulated human endothelial cells [Articolo su rivista]
Lo Vasco, V. R.; Leopizzi, M.; Di Maio, V.; Di Raimo, T.; Cesa, S.; Masci, A.; Rocca, C. D.
abstract

Endothelial cells (EC) act as leading actors in angiogenesis. Understanding the complex network of signal transduction pathways which regulate angiogenesis might offer insights in the regulation of normal and pathological events, including tumours, vascular, inflammatory and immune diseases. The effects of olive oil and of Blueberry extracts upon the phosphoinositide (PI)-specific phospholipase C (PLC) enzymes were evaluated both in quiescent and inflammatory stimulated human umbilical vein EC (HUVEC) using molecular biology (multiliquid bioanalysis) and immunofluorescence techniques. Oleuropein significantly increased the number of surviving HUVEC compared to untreated controls, suggesting that it favours the survival and proliferation of EC. Our results suggest that Oleuropein might be useful to induce EC proliferation, an important event during angiogenesis, with special regard to wound healing. Blueberry extracts increased the number of surviving HUVEC, although the comparison to untreated controls did not result statistically significant. Lipopolysaccharide (LPS) administration significantly reduced the number of live HUVEC. LPS can also modify the expression of selected PLC genes. Adding Blueberry extracts to LPS treated HUVEC cultures did not significantly modify the variations of PLC expression induced by LPS. Oleuropein increased or reduced the expression of PLC genes, and statistically significant results were identified for selected PLC isoforms. Oleuropein also modified the effects of LPS upon PLC genes’ expression. Thus, our results corroborate the hypothesis that Oleuropein owns anti-inflammatory activity. The intracellular localization of PLC enzymes was modified by the different treatments we used. Podosome-like structures were observed in differently LPS treated HUVEC.

2017 - Wounds difficult to heal: An effective treatment strategy [Articolo su rivista]
Capoano, R.; Businaro, R.; Tesori, M. C.; Donello, C.; Lombardo, Federica; Lo Vasco, Vincenza Rita; Capriotti, L.; Corsi, Mariangela; Raimo, T. D.; Leopizzi, M.; Salvati, B.; Ricci, Serafino
abstract

Objective: Treatment of wounds difficult to heal concerns 50% of the elderly population in Italy and is therefore a relevant social burden. The present study shows how the treatment with autologous leuco-platelets reduces the healing time of wounds improving the functional recovery. Patients and Methods: Patients (n=100) with ulcers of the legs were divided in two groups: 1) 50 patients treated with conventional therapies; 2) 50 patients treated with autologous leuco-platelet concentrate (LPC) and hyaluronic acid (HIAFF, Hyalofill-F®) as a scaffold. Results: After 2 months, a 49% reduction in wound area was observed in the second group and in about 65% wound reduction was achieved in 15 days (4 LPC dressings). In contrast, patients treated by conventional therapies, showed a longer healing time and a greater percentage of failures. Morphometric analysis of biopsy samples obtained from the edge as well as from the bottom of the lesions obtained from the LPC group, detected an abundant presence of neoformed capillaries, characterized by a cubic, “reactive endothelium”, close to the site of LPC infiltration. Conclusion: These results suggest that healing was promoted not only by limiting bacterial infections but also by the release of chemotactic and proangiogenic factors from leukocytes and platelets, improving the neoformation of capillaries.

2016 - Different expression and subcellular localization of Phosphoinositide-specific Phospholipase C enzymes in differently polarized macrophages [Articolo su rivista]
DI RAIMO, Tania; Leopizzi, Martina; Mangino, Giorgio; DELLA ROCCA, Carlo; Businaro, Rita; Longo, Lucia; LO VASCO, VINCENZA RITA
abstract

2016 - Expression and localization of phosphoinositide-specific phospholipase C in polarized macrophages [Abstract in Atti di Convegno]
Di Raimo, T; Businaro, R; Leopizzi, M; Della Rocca, C; LO VASCO, VINCENZA RITA
abstract

2016 - Relapsing polychondritis: a clinical update [Articolo su rivista]
Longo, Lucia; Greco, Antonio; Rea, Andrea; Lo Vasco, Vincenza Rita; De Virgilio, Armando; De Vincentiis, Marco
abstract

2016 - Signal transduction pathways in the clinical approach: where are we going? [Articolo su rivista]
LO VASCO, VINCENZA RITA
abstract

invited editorial

2016 - U-73122 reduces the cell growth in cultured MG-63 ostesarcoma cell line involving Phosphoinositide-specific Phospholipases C [Articolo su rivista]
LO VASCO, VINCENZA RITA; Leopizzi, Martina; DI MAIO, Valeria; DELLA ROCCA, Carlo
abstract

2015 - Adipokines and their Involvement as a target of new drugs [Articolo su rivista]
DI RAIMO, Tania; Azzara, Gabriella; Corsi, Mariangela; Cipollone, D; LO VASCO, VINCENZA RITA; Businaro, Rita
abstract

Globesity is referred to a global epidemic of obesity, affecting millions of individuals. Molecules released by the enlarged adipose tissue, most of which are pro-inflammatory, have been named adipokines. The present review deals with function, molecular targets and the potential clinical relevance of adipokines. Currently, more than 600 adipokines have been identified, many of them, including leptin, visfatin, resistin as well as Retinol Binding Protein4 may serve as informative markers for metabolic and cardiovascular diseases and play important roles in glucose homeostasis, insulin sensitivity as well as metabolic regulation of energy expenditure. Adiponectin on the contrary exerts anti-inflammatory and insulin sensitizing activity. Adiponectin has additional anti-atherogenic effects and low adiponectin serum concentrations are associated with increased risk for cardiovascular diseases. The understanding of the role of adipokines has provided a wealth of information that has opened great opportunities for new therapeutic advances. Adiponectin may be the most prominent example for the potential use of an adipokine in the treatment of obesity and obesity-associated metabolic diseases. In many studies, administration of recombinant adiponectin results in improved insulin sensitivity, increased insulin secretion and beneficial effects on body weight and hyperglycemia. Up-regulation of adiponectin/adiponectin receptors or enhancing adiponectin receptor function may be an interesting therapeutic strategy for obesity-linked insulin resistance. Moreover, the therapeutic use of combined amylin/leptin agonism (with pramlintide and metreleptin) demonstrated a significant weight-lowering effect in obese subjects. Therefore, adipokines may be clinically relevant either as therapeutic tools or as target in the treatment of obesity related diseases.

2015 - Ezrin-related phosphoinositide pathway modifies RhoA and Rac1 in human osteosarcoma cell lines [Articolo su rivista]
Lo Vasco, Vincenza Rita; Leopizzi, Martina; Della Rocca, Carlo
abstract

2015 - Impairment and reorganization of the phosphoinositide-specific phospholipase C enzymes in suicide brains [Articolo su rivista]
LO VASCO, VINCENZA RITA; Leopizzi, Martina; DELLA ROCCA, Carlo; Fais, P; Montisci, M; Cecchetto, G.
abstract

2014 - Degradation of Silicone Rubber Causes Provox 2 Voice Prosthesis Malfunctioning [Articolo su rivista]
Fusconi, Massimo; Anna Rita, Taddei; Gallo, Andrea; Michela, Conte; De Virgilio, Armando; Greco, Antonio; Lo Vasco, Vincenza Rita; Gian Franco, Macri; Roma, Rocco; Fabrizio, Volpini; Anna Teresa, Benincasa; De Vincentiis, Marco
abstract

2014 - Ezrin silencing remodulates the expression of Phosphoinositide-specific Phospholipase C enzymes in human osteosarcoma cell lines [Articolo su rivista]
Lo Vasco, Vincenza Rita; Leopizzi, Martina; Puggioni, C.; Della Rocca, Carlo
abstract

Ezrin, a protein belonging to the Ezrin, radixin and moesin (ERM) family, was engaged in the metastatic spread of osteosarcoma. The Protein 4.1, Ezrin, radixin, moesin (FERM) domain of Ezrin binds the membrane Phosphatydil inositol (4,5) bisphosphate (PIP2), a crucial molecule belonging to the Phosphoinositide (PI) signal transduction pathway. The cytoskeleton cross-linker function of Ezrin largely depends on membrane PIP2 levels, and thus upon the activity of related enzymes belonging to the PI-specific phospholipase C (PI-PLC) family. Based on the role of Ezrin in tumour progression and metastasis, we silenced the expression of Vil2 (OMIM *123900), the gene which codifies for Ezrin, in cultured human osteosarcoma 143B and Hs888 cell lines. After Ezrin silencing, the growth rate of both cell lines was significantly reduced and morphogical changes were observed. We also observed moderate variations both of selected PI-PLC enzymes within the cell and of expression of the corresponding PLC genes. In 143B cell line the transcription of PLCB1 decreased, of PLCG2 increased and of PLCE differed in a time-dependent manner. In Hs888, the expression of PLCB1 and of PLCD4 significantly increased, of PLCE moderately increased in a time dependent manner; the expression of PLCG2 was up-regulated. These observations indicate that Ezrin silencing affects the transcription of selected PLC genes, suggesting that Ezrin might influence the expression regulation of PI-PLC enzymes.

2014 - Fibroblast growth factor acts upon the transcription of phospholipase C genes in human umbilical vein endothelial cells [Articolo su rivista]
Lo Vasco, Vincenza Rita; Leopizzi, Martina; Puggioni, Chiara; Della Rocca, Carlo; Businaro, Rita
abstract

Besides the control of calcium levels, the phosphoinositide-specific phospholipases C (PI-PLCs), the main players in the phosphoinositide signalling pathway, contribute to a number of cell activities. The expression of PI-PLCs is strictly tissue specific and evidence suggests that it varies under different conditions, such as tumour progression or cell activation. In previous studies, we obtained a complete panel of expression of PI-PLC isoforms in human umbilical vein endothelial cells (HUVEC), a widely used experimental model for endothelial cells (EC), and demonstrated that the expression of the PLC genes varies under inflammatory stimulation. The fibroblast growth factor (FGF) activates the PI-PLC γ1 isoform. In the present study, PI-PLC expression in FGF-treated HUVEC was performed using RT-PCR, observed 24 h after stimulation. The expression of selected genes after stimulation was perturbed, suggesting that FGF affects gene transcription in PI signalling as a possible mechanism of regulation of its activity upon the AkT-PLC pathway. The most efficient effects of FGF were recorded in the 3-6-h interval. To understand the complex events progressing in EC might provide useful insights for potential therapeutic strategies. The opportunity to manipulate the EC might offer a powerful tool of considerable practical and clinical importance. © 2013 Springer Science+Business Media New York.

2014 - Hunting the Risk. NPY and ACE Polymorphisms as Predictors of Cardiovascular Diseases: Case Report and Review of the Literature [Articolo su rivista]
LO VASCO, VINCENZA RITA; Businaro, Rita; Massoni, Francesco; Borghini, G; Corsi, Mariangela; Simeone, C; Ricci, Serafino
abstract

2014 - Hunting the risk NPY and ACE Polymorphisms as predictors of cardiovascular diseases: case report and review of the literature [Capitolo/Saggio]
LO VASCO, VINCENZA RITA; Businaro, R; Massoni, F; Borghini, G; Corsi, M; Simeone, C; Ricci, S.
abstract

Many research efforts were addressed to identify individuals at high risk for multifactorial diseases, such as cardiovascular alterations and related diseases. Great interest was paid to investigate the genetic liability in multifactorial illnesses. The prognosis of high-risk patients might be greatly ameliorated using genetic predisposition risk factors, such as the polymorphisms of neuropeptide Y (NPY) and Angiotensin converting enzyme (ACE) genes. Epidemiologic results suggest that selected polymorphisms of both NPY and ACE might be helpful to improve the evaluation of patients, offering a powerful prognostic tool and paving the way to novel molecular therapeutic strategies. We present a case report of a male, sudden-death from myocardial infarction, presenting with left ventricular hypertrophy. The patient carried polymorphisms of ACE and NPY genes, respectively ACE genotype ID and NPY genotype T-399C, actually considered as risk factors.

2014 - Neuropeptide Y significantly reduces the expression of PLCB2, PLCD1 and moderately decreases selected PLC genes in endothelial cells [Articolo su rivista]
LO VASCO, VINCENZA RITA; Leopizzi, Martina; Puggioni, C.; Della Rocca, Carlo; Businaro, Rita
abstract

Endothelial cells (EC) are the first elements exposed to mediators circulating in the bloodstream, and react to stimulation with finely tuned responses mediated by different signal transduction pathways, leading the endothelium to adapt. Neuropeptide Y (NPY), the most abundant peptide in heart and brain, is mainly involved in the neuroendocrine regulation of the stress response. The regulatory roles of NPY depend on many factors, including its enzymatic processing, receptor subtypes and related signal transduction systems, including the Phosphoinositide (PI) pathway and related phospholipase C (PI-PLC) family of enzymes. The panel of expression of PI-PLC enzymes differs comparing quiescent versus differently stimulated human EC. Growing evidences indicate that the regulation of the expression of PLC genes, which codify for PI-PLC enzymes, might act as an additional mechanism of control of the PI signal transduction pathway. NPY was described to potentiate the activation of PI-PLC enzymes in different cell types, including EC. In the present experiments, we stimulated human umbilical vein EC using different doses of NPY in order to investigate a possible role upon the expression PLC genes. NPY reduced the overall transcription of PLC genes, excepting for PLCE. The most significant effects were observed for PLCB2 and PLCD1, both isoforms recruited by means of G-proteins and G-protein-coupled receptors. NPY behavior was comparable to other PI-PLC interacting molecules that, beside the stimulation of phospholipase activity, also affect the upcoming enzymes’ production acting upon gene expression. That might represent a mode to regulate the activity of PI-PLC enzymes after activation.

2014 - Phosphoinositide-Specific Phospholipase C Enzymes and Cognitive Development and Decline [Capitolo/Saggio]
LO VASCO, VINCENZA RITA
abstract

The development of the mammalian nervous system is a tightly regulated and complex process, which involves a number of signal transduction pathways, which control the cascade of events, both spatially and temporally. Complex modifications of the structural and functional bases of the activities of the nervous system also occur in the cognitive decline often observed during aging. The Phosphoinositide (PI) signal transduction pathway, which contributes to regulate the calcium levels by means of converting enzymes, such as the Phosphoinositide-specific Phospholipase C (PLC) family, interacts at different hierarchy of control with a number of different molecules and/or pathways involved in neural development, neurogenesis and maintenance of the synaptic plasticity. The PI pathway was suggested to be involved in the complex mechanism of memory, crucial and strictly correlated to learning abilities. Specific roles were also suggested for PLC isoforms, on the basis of numerous evidences indicating the involvement in diseases which affect the nervous system, with special regard to the cognitive impairment. The nature, meaning, and developmental period of PLC involvement in cognitive development and decline are still largely unclear and will require further studies.

2014 - Silencing of phosphoinositide-specific phospholipase C ε remodulates the expression of the phosphoinositide signal transduction pathway in human osteosarcoma cell lines [Articolo su rivista]
Lo Vasco, Vincenza Rita; Leopizzi, Martina; Stoppoloni, D.; Della Rocca, Carlo
abstract

Ezrin, a member of the ezrin-radixin-moesin family, is involved in the metastatic spread of osteosarcoma. Ezrin binds phosphatydil inositol-4,5-bisphosphate (PIP2), a crucial molecule of the phosphoinositide signal transduction pathway. PIP2 levels are regulated by phosphoinositide-specific phospholipase C (PI-PLC) enzymes. PI-PLCε isoform, a well-characterized direct effector of rat sarcoma (RAS), is at a unique convergence point for the broad range of signaling pathways that promote RAS GTPase-mediated signalling. Materials and Methods. By using molecular biology methods and microscopic analyses, we analyzed the expression of ezrin and PLC genes after silencing of PLCE (OMIM *608414) in 143B and Hs888 cell lines. The growth rate of the cells was slowed, and the expression of ezrin, PLCB1, PLCG2 and PLCD4 was significantly modified. Ezrin displacement from the plasma membrane was observed. The present results corroborate the hypothesis that ezrin and the PI signal transduction system are involved in a common network. Copyright© 2014 International Institute of Anticancer Research (Dr. John G. Delinassios), All rights reserved.

2014 - The involvement of Phosphoinositide signal transduction pathway in angiogenesis [Relazione in Atti di Convegno]
LO VASCO, VINCENZA RITA
abstract

2013 - Altered Cytokine and BDNF Levels in Autism Spectrum Disorder [Articolo su rivista]
Ricci, Serafino; Businaro, Rita; Ippoliti, Flora; LO VASCO, VINCENZA RITA; Massoni, Francesco; Onofri, E.; Troili, G. M.; Pontecorvi, V.; Morelli, M.; Rapp Ricciardi, M.; Archer, T.
abstract

The contribution of neuroimmune functioning and brain-derived neurotrophic factor (BDNF) to functional dysregulation in autism spectrum disorder was assessed in 29 patients under treatment in two specialized centers of Basilicata (Chiaromonte and Matera), Southern Italy, through analysis of serum levels of cytokines and BDNF. Elevated levels of the pro-inflammatory cytokine, including interleukin-1, interleukin-6, interleukin-12, interleukin-23, tumor necrosis factor-α and BDNF were observed, regardless of age and gender. Comparisons were made with age- and gender-related healthy controls. The present findings reinforce current notions regarding immunoexcitotoxic mechanisms contributing to the pathophysiology of autistic disorder.

2013 - Analysis of Phosphoinositide-specific phospholipase C enzymes gene expression in human osteosarcoma [Relazione in Atti di Convegno]
LO VASCO, VINCENZA RITA; Leopizzi, M; Della Rocca, C
abstract

2013 - Expression of Phosphoinositide-Specific Phospholipase C Enzymes in Human Skin Fibroblasts [Articolo su rivista]
Lo Vasco, Vincenza Rita; Leopizzi, Martina; Chiappetta, Caterina; Puggioni, C.; Di Cristofano, Claudio; Della Rocca, Carlo
abstract

Fibroblasts are involved in a number of functions regulated by different signal transduction pathways, including the phosphoinositide (PI) signaling system and related converting enzymes, such as phosphoinositide-specific phospholipase C (PI-PLC). The PI-PLC family comprises crucial effector enzymes in the PI signal transduction pathway. Once activated, PI-PLC cleaves an important membrane PI, the phosphatidylinositol (4,5) bisphosphate into inositol trisphosphate and diacylglycerol-both are crucial molecules in the transduction of signals. The activity of selected PI-PLC enzymes was reported in fibroblasts, although the complete panel of expression was not available. Each cell type expresses a group of selected PI-PLC isoforms, and knowledge of the panel of expression is a necessary and preliminary tool to address further studies. In the present study, we delineated the expression panel of PI-PLC enzymes in human skin fibroblasts. PI-PLC beta 1, PI-PLC beta 3, PI-PLC beta 4, PI-PLC gamma 1, PI-PLC gamma 2, PI-PLC delta 1, PI-PLC delta 3, PI-PLC delta 4, and PI-PLC epsilon were expressed. PI-PLC beta 1 was weakly expressed, PI-PLC delta 4 was inconstantly expressed, and PI-PLC gamma 2 was weakly expressed.

2013 - Expression of Phosphoinositide-specific phospholipase C enzymes in human osteosarcoma cell lines [Articolo su rivista]
LO VASCO, VINCENZA RITA; Leopizzi, Martina; Chiappetta, Caterina; Puggioni, Chiara; Di Cristofano, Claudio; Della Rocca, Carlo
abstract

The definition of the number and nature of signal transduction pathways networking in the pathogenesis of osteosarcoma raised great interest. Intracellular calcium ions are important second messengers implicated in the control of cell death. The calcium concentration is regulated by signal transduction pathways, including the Phosphoinositides (PI) signaling. Phosphatydil inositol (4,5) bisphosphate (PIP2) is critical for many cellular activities. The levels of PIP2 are regulated by means of Phosphoinositide- specific Phospholipase C (PI-PLC) family of enzymes. We delineated the panel of expression of PI-PLC enzymes in four human osteosarcoma cell lines. In MG-63 cell line, PI-PLC β1, β2, β3, β4, γ1, γ2, δ1, δ3 and ε resulted expressed. In 143B cell line, PI-PLC β1, β2, β3, β4, γ1, γ2, δ1, δ3 and ε were expressed. In SaOS-2 cell line, PI-PLC β1, β3, β4, γ1, γ2, δ1, δ3, ε and η1. In Hs888 cell line, PI-PLC β1, β3, β4, γ1, δ1, δ3, δ4, ε and η1 the administration of U-73122 to cultures briefly modifies the levels of PI-PLC transcripts. The obtained complete expression panel of PI-PLC isoforms will be a useful tool for further functional studies about the role of the PI signal transduction pathway in osteosarcoma. © 2013 The International CCN Society.

2013 - Expression of phospholipase C genes in cultured endothelial cells [Relazione in Atti di Convegno]
LO VASCO, VINCENZA RITA; Leopizzi, M; Azzara, G; Cannizzaro, C; Dante, D; Della Rocca, C; Businaro, R
abstract

2013 - Is Montgomery Tracheal Safe-T-Tube Clinical Failure Induced by Biofilm? [Articolo su rivista]
Fusconi, Massimo; LO VASCO, VINCENZA RITA; A., Delfini; DE VIRGILIO, Armando; A. R., Taddei; C., Vassalli; Conte, Michela; F., Del Sette; A. T., Benincasa; DE VINCENTIIS, Marco
abstract

2013 - Lypopolysaccharide Downregulates the Expression of Selected Phospholipase C Genes in Cultured Endothelial Cells [Articolo su rivista]
Lo Vasco, Vincenza Rita; Leopizzi, Martina; Chiappetta, Caterina; Puggioni, C.; Rocca, C.; Della Rocca, Carlo; Polonia, P.; Businaro, Rita
abstract

The signaling system of phosphoinositides (PI) is involved in a variety of cell and tissue functions, including membrane trafficking, ion channel activity, cell cycle, apoptosis, differentiation, and cell and tissue polarity. Recently, PI and related molecules, such as the phosphoinositide-specific phospholipases C (PI-PLCs), main players in PI signaling were supposed to be involved in inflammation. Besides the control of calcium levels, PI-PLCs contribute to the regulation of phosphatydil-inositol bisphosphate metabolism, crucial in cytoskeletal organization. The expression of PI-PLCs is strictly tissue specific and evidences suggest that it varies under different conditions, such as tumor progression or cell activation. In a previous study, we obtained a complete panel of expression of PI-PLC isoforms in human umbilical vein endothelial cells (HUVEC), a widely used experimental model for endothelial cells. In the present study, we analyzed the mRNA concentration of PI-PLCs in lipopolysaccharide (LPS)-treated HUVEC by using the multiliquid bioanalyzer methodology after 3, 6, 24, 48, and 72 h from LPS administration. Marked differences in the expression of most PI-PLC codifying genes were evident.

2013 - PLCB1 deletions in mood disorders [Relazione in Atti di Convegno]
LO VASCO, VINCENZA RITA; Polonia, P
abstract

2013 - Phosphoinositide Signal Transduction Pathway and Major Depression [Capitolo/Saggio]
LO VASCO, VINCENZA RITA
abstract

Mood disorders, including major depression (MD), overall constitute a major medical need. In fact, mood disorders require chronic treatments, lathough not effective in all patients. Multiple deficits, including cell atrophy and loss, were described in limbic and cortical brain regions of patients affected with mood disorders and in experimental animal models. A number of changes in gene expression and activity was described in brains of mood disorder affected patients. Therapies act reciprocally regulating many of these changes. Antidepressant and mood stabilizing therapies restore these deficits by reestablishing proper patterns of gene expression and function. Different signal transduction pathways play a role in the pathogenesis of mood disorders, namely the cyclic‐AMP, phosphoinositides (PI), mitogen‐activated protein kinase, and glycogen synthase kinase cascades. Although significant progresses have been achieved in studying the signal transduction pathways possibly involved in mood disorders, their reciprocal interconnection and the effect of alterations in human brain, many issues remain to be addressed. Knowledge of these intriguing aspects might help to clarify the pathogenesis of MD, widening the panel of available therapeutic tools.

2013 - Phosphoinositide-specific Phospholipase C b1 gene deletion in bipolar disorder affected patient [Articolo su rivista]
Lo Vasco, V. R.; Longo, L.; Polonia, P.
abstract

The involvement of phosphoinositides (PI) signal transduction pathway and related molecules, such as the Phosphoinositide-specific Phospholipase C (PI-PLC) enzymes, in the pathophysiology of mood disorders is corroborated by a number of recent evidences. Our previous works identified the deletion of PLCB1 gene, which codifies for the PI-PLC beta1 enzyme, in 4 out 15 patients affected with schizophrenia, and no deletion both in major depression affected patients and in normal controls. By using interphase fluorescent in situ hybridization methodology, we analyzed PLCB1 in paraffin embedded samples of orbito-frontal cortex of 15 patients affected with bipolar disorder. Deletion of PLCB1 was identified in one female patient.

2013 - Phosphoinositide-specific Phospholipases C play might play a role in cognitive disorders [Relazione in Atti di Convegno]
LO VASCO, VINCENZA RITA; Longo, L; Dante, D
abstract

2013 - The Phosphoinositide Signal Transduction Pathway In Endometriosis: A Potential Prognostic And Therapeutic Tool [Capitolo/Saggio]
LO VASCO, VINCENZA RITA
abstract

Uncontrolled calcium levels in the cell alter proteins function, apoptosis regulation, as well as proliferation, secretion and contraction. Calcium levels are tightly regulated by signal transduction pathways, including the Phosphoinositide (PI) system. A great interest was paid to PIs for their role in mammalian reproduction. Reports from the ‘80s indicated that the PI signal transduction pathway plays a role in the endocrine regulation of endometrium. PI-PLC enzymes were recently suggested to be involved in endometriosis. Recent studies demonstrated that the expression differed in normal endometrium with respect to endometriosis tissue. Remarkably, recent evidences indicated that PI-PLC isoforms might be involved in the inflammation cascade in different cytotypes. That suggests that PI-PLC enzymes might be related to the inflammatory cascade claimed in the pathogenesis of endometriosis.

2013 - The Phosphoinositide network in human central nervous system development [Relazione in Atti di Convegno]
LO VASCO, VINCENZA RITA
abstract

2013 - The Phosphoinositide signal transduction Pathway and the development of human nervous system [Relazione in Atti di Convegno]
LO VASCO, VINCENZA RITA
abstract

The development of nervous system is tightly regulated by a network of interconnected signal transduction pathways. The extensive crosstalk among different signal transduction systems deserves great attention. In fact, understanding the timing of the cascade of events regulating the development of the nervous system might open the way to novel therapeutic strategies. In the last 20 years, great interest was paid to the Phosphoinositide (PI) signal transduction pathway and related Phosphoinositide-specific phospholipids C (PI-PLC) family of converting enzymes, which contribute to the regulation of intracellular calcium levels. Beside their well-known role in the metabolism of calcium, PI-PLC enzymes interact with a number of molecules belonging to other signal transduction pathways, contributing to the peculiar and complex network in the developing nervous system. In the present communication, the connection of PI signalling and further transduction pathways acting during neural development will be analyzed, with special regard to the role of PI-PLC family of enzymes.

2012 - Deletion of PLCB1 gene in schizophrenia affected patients [Articolo su rivista]
LO VASCO, V.; G, Cardinale; P, Polonia
abstract

A prevalence of 1% in the general population and approximately 50% concordance rate in monozygotic twins was reported for schizophrenia, suggesting that genetic predisposition affecting neurodevelopmental processes might combine with environmental risk factors. A multitude of pathways seems to be involved in the etiology and/or pathogenesis of schizophrenia, including dopaminergic, serotoninergic, muscarinic and glutamatergic signaling. The phosphoinositide signal transduction system and related phosphoinositide-specific phospholipase C (PI-PLC) enzymes seem to represent a point of convergence in these networking pathways during the development of selected brain regions. The existence of a susceptibility locus on the short arm of chromosome 20 moved us to analyze PLCB1, the gene codifying for PI-PLC β1 enzyme, which maps on 20p12. By using interphase fluorescent in situ hybridization (I-FISH) methodology, we found deletions of PLCB1 in orbito-frontal cortex samples of schizophrenia affected patients.

2012 - Expression of phosphoinositide-specific phospholipase C enzymes in normal endometrium and in endometriosis [Articolo su rivista]
Lo Vasco, Vincenza Rita; Leopizzi, Martina; Chiappetta, Caterina; Businaro, Rita; Polonia, Patrizia; Della Rocca, Carlo; Litta, Pietro
abstract

To delineate the panel of expression of phosphoinositide-specific phospholipase C (PI-PLC) signaling enzymes in normal endometrium and in endometriosis.

2012 - IL-1 and IL-23 in amniotic fluids of ultrasound-detected aortic intima/media thickness and growth retardation [Relazione in Atti di Convegno]
Cosmi, E.; Visentin, S.; Lo Vasco, V. R.
abstract

2012 - IL-1 beta and IL-23 in amniotic fluids of ultrasound-detected aortic intima/media thickness and growth retardation [Articolo su rivista]
Lo Vasco, Vincenza Rita; Cosmi, E.; Visentin, S.; Di Raimo, Tania; Salmaso, R.; Zanardo, V.; Trevisanuto, D.; Businaro, Rita
abstract

Intrauterine growth restriction (IUGR) and/or neonatal low birth weight are often associated with increased intima/media thickness of the abdominal aortic wall (aIMT). Several studies in children suggested that aIMT might be related to inflammation, probably indicating an early stage of adulthood diseases, such as atherosclerosis. Our previous study performed on the abdominal aortic wall of a stillbirth presenting with IUGR and aIMT suggested an association among IUGR, aIMT, and inflammation, also highlighting the presence of fibroblastoid cells, which are thought to represent peculiar elements of the pre-atherosclerotic lesions. These observations led us to analyze two cytokines involved in the inflammation cascade, IL-1 beta and IL-23, in amniotic fluid samples of IUGR fetuses and small-for-gestational-age newborns presenting with aIMT and in normal controls. Our results indicate that IL-23, but not IL-1 beta, concentrations differed in the groups analyzed. Therefore, IL-23, a regulatory element that bridges the innate and adaptive arms of the immune system, might be involved in the inflammatory process observed in fetal aIMT. (C) 2011 Elsevier Ireland Ltd. All rights reserved.

2012 - Molecular Cytogenetic Interphase analysis of Phosphoinositide-specific Phospholipase C <beta>1 gene in paraffin- embedded brain samples of major depression patients [Articolo su rivista]
Lo Vasco, V. R.; Polonia, P.
abstract

Mood disorders represent amajormedical need, as their chronic treatments are not effective in all patients. Literature data suggested that phosphoinositides (PI) signal transduction pathway and related molecules such as the Phosphoinositide-specific Phospholipase C (PI-PLC) enzymes, might be involved in the pathophysiology of mood disorders, including major depression. By using interphase fluorescent in situ hybridization methodology,we analyzed PLCB1 gene,which codifies for the PI-PLC β1 enzyme, in paraffin embedded samples of orbito-frontal cortex of 15 patients affected with major depression and in 15 normal controls. No deletions of PLCB1 were identified with the methodology used, which allows to exclude wide gene deletions. The results, the technical aspects of the FISH methodology, and its limitations are discussed.

2012 - Phosphoinositide network in human central nervous system development [Relazione in Atti di Convegno]
LO VASCO, VINCENZA RITA
abstract

2012 - Phosphoinositide pathway and the signal transduction network in neural development [Articolo su rivista]
Lo Vasco, V. R.
abstract

The development of the nervous system is under the strict control of a number of signal transduction pathways, often interconnected. Among them, the phosphoinositide (PI) pathway and the related PI-specific phospholipid C (PI-PLC) family of enzymes have been attracting much attention. Besides their well-known role in the regulation of intracellular calcium levels, PI-PLC enzymes interact with a number of molecules belonging to further signal transduction pathways, contributing to a specific and complex network in the developing nervous system. In this review, the connections of PI signalling with further transduction pathways acting during neural development are discussed, with special regard to the role of the PI-PLC family of enzymes.

2012 - Role of the phosphoinositide signal transduction pathway in the endometrium [Articolo su rivista]
Lo Vasco, V. R.
abstract

The regulation of calcium concentration triggers physiological events in all cell types. Unregulated elevation in calcium concentrations is often cytotoxic. In fact, uncontrolled calcium levels alter proteins' function, apoptosis regulation, as well as proliferation, secretion and contraction. Calcium levels are tightly regulated. A great interest was paid to signal transduction pathways for their role in mammalian reproduction. The role of phosphoinositide (PI) signal transduction pathway and related phosphoinositide-specific phospholipase C (PI-PLC) enzymes in the regulation of calcium levels was actively studied and characterized. However, the role of PI signaling and PI-PLC enzymes in the endometrium is far to be completely highlighted. In the present review the role of PI, the expression of selected PI-PLC enzymes and the crosstalk with further signaling systems in the endometrium will be discussed.

2012 - The phosphoinositides signal transduction pathway in astrocytes [Capitolo/Saggio]
Lo Vasco, V. R.
abstract

Signal transduction from plasma membrane to cell nucleus is a complex process depending on various components including lipid signaling molecules. Phosphoinositides (PI) constitute an important signaling system. Enzymes related to the PI signal transduction pathway may act at cell periphery, plasma membrane or nuclear level. The PI cycle was also hypothesized to be involved in neuronal as well as glial activities. Many evidences support the hypothesis that the PI cycle is involved in astrocytes activation during the neurodegeneration process. Phosphoinositide-specific phospholipase C (PI-PLC) family of enzymes is crucial in PI signaling system. In fact, PI-PLC enzymes regulate the spatial and temporal balance of PI. Thirteen mammalian PI-PLC isoforms were identified, divided into six sub-families on the basis of amino acid sequence, domain structure and mechanism of recruitment in response to activated receptors: β(1–4), γ(1, 2), δ(1, 3, 4), ϵ(1), ζ(1), and η(1-2). Different expression of the isoforms was described in pathological cells with respect to the corresponding normal counterparts. The expression panel of PI-PLC isoforms varies under different conditions, such as tumoral progression or inflammatory activation, with respect to the quiescent astrocytes counterpart. These observations suggest that in the nervous system the fine regulation of PI-PLC isoforms play a role in the activation of the glia and in the inflammation processes.

2012 - The role of Phosphoinositide-specific Phospholipase C family of enzymes in neural development [Relazione in Atti di Convegno]
LO VASCO, VINCENZA RITA
abstract

2011 - 1p36.32 rearrengements and the role of PI-PLC 2 in nervous tumours [Articolo su rivista]
LO VASCO, Vr.
abstract

Deletions in the distal region of the short arm of chromosome 1 (1p36) are widely diffuse, both in congenital 1p36 Deletion Syndrome and as somatic abnormalities in tumours. Rearrangements in 1p36 have been described in a broad spectrum of human neoplasias in addition to other chromosomal abnormalities. In neuroblastomas, wide hemizygous deletions in 1p36.23–1p36.32 have been described suggesting that the 1p36 region contains a tumour-suppressor gene involved in malignancy. A role for phosphoinositide (PI)-specific phospholipase C (PLC) ETA2, whose gene maps on 1p36.32, was suggested. PI-PLC ETA2 belongs to a family of enzymes related to the phosphoinositide signalling pathway, which provide an important intracellular signalling system involved in a variety of cell functions such as hormone secretion, neurotransmitter signal transduction, cell growth, membrane trafficking, ion channel activity, regulation of the cytoskeleton, cell cycle control and apoptosis. Expression of PI-PLC g2 occurs after birth and continues throughout the life. Synapse formation occurs during a short period of postnatal development. Thus, it is likely that PI-PLC ETA2 acts in formation and maintenance of the neuronal network in the brain. The fact that PI-PLC ETA2, a highly neuronspecific isozyme, is abundantly expressed in the postnatal brain suggests the importance of PI-PLC g2 in formation and maintenance of the neuronal network in the postnatal brain. Further studies are required to verify the possible involvement of PI-PLC ETA2 mutation/deletion in central nervous tumour tissues presenting abnormalities of the 1p36 chromosomal band.

2011 - Abdominal aorta pathologic findings in stillbirth fetuses with intrauterine growth restriction [Relazione in Atti di Convegno]
Cosmi, E.; Zanardo V, V.; Visentin, S.; Salmaso, R.; Lo Vasco, V.
abstract

2011 - Expression of phosphoinositide-specific phospholipase C isoforms in human umbilical vein endothelial cells [Articolo su rivista]
Lo Vasco, Vincenza Rita; Pacini, Luca; Di Raimo, Tania; D'Arcangelo, D.; Businaro, Rita
abstract

Aims The signalling system of phosphoinositides (PIs) is involved in a number of cell and tissue functions including membrane trafficking, ion channel activity, cell cycle, apoptosis, differentiation and cell and tissue polarity. Recently, a role in cell migration was hypothesised for PI and related molecules including the phosphoinositide-specific phospholipases C (PI-PLCs), main players in PI signalling. The expression of PI-PLCs is tissue-specific and evidence suggests that it varies under different conditions such as tumour progression or cell activation. In order to obtain a complete picture, the expression of all PI-PLC isoforms was analysed in human endothelial cells (EC). Methods Using molecular biology methods (RT-PCR), the expression of PI-PLC isoforms was analysed in human umbilical vein endothelial cells (HUVEC), a widely used experimental model for human EC. Results All the PI-PLC isoforms except PI-PLC beta 1, PI-PLC epsilon and PI-PLC zeta were expressed in HUVEC. Conclusions The growing interest in the complex cascade of events occurring in angiogenesis will provide useful insights for therapeutic strategies. The expression of PI-PLC isoforms in HUVEC is a useful tool for further studies directed to understanding their role in angiogenesis. However, although HUVEC represent a widely used experimental model for human macrovascular EC, limitations remain in that they cannot fully represent the metabolic properties and interactions of the EC distributed in the entire organism.

2011 - Fetal aorta wall inflammation in ultrasound-detected aortic intima/media thickness and growth retardation [Articolo su rivista]
LO VASCO, VINCENZA RITA; Salmaso, Roberto; Zanardo, Vincenzo; Businaro, Rita; Visentin, Silvia; Cosmi, Erich
abstract

Several studies have reported that fetuses with intrauterine growth restriction (IUGR) and infants with low birth weight present increased intima/media thickness (aIMT) of the abdominal aorta wall compared with fetuses and infants appropriate for gestational age (AGA). Evidence suggested that aIMT might be related to inflammation, probably indicating a very early stage of future adulthood disease, such as atherosclerosis. We aimed to investigate histological findings in the abdominal aorta wall of one IUGR stillbirth in which ultrasound had detected aIMT. Microscopy observations of the abdominal aorta wall confirmed the intima thickening and detected condensation of the elastic fibers forming an evident internal elastic membrane and presence of inflammatory elements, such as macrophages, activated endothelial cells, and fibroblastoid cells. The present study highlights that IUGR associated with aIMT is related to inflammation, which might represent a very early sign of future adult lesions. (C) 2011 Elsevier Ireland Ltd. All rights reserved.

2011 - Role of Phosphoinositide-specific Phospholipase C eta2 in isolated and syndromic mental retardation [Articolo su rivista]
Lo Vasco, Vincenza Rita
abstract

Deletions in the distal region of the short arm of chromosome 1 (1p36) are widely diffuse, both as somatic abnormalities in tumors and as constitutive in the congenital 1p36 deletion syndrome. The deletion size varies from 1.5 to 10 Mb, with common breakpoints located from 1p36.13 to 1p36.33. Patients bearing constitutional deletion of a smaller region, 1p36.3, present with a number of features, including mental retardation. The gene PLCH2, codifying for the phosphoinositide-specific phospholipase C (PI-PLC) η2, maps on the 1p36.32 region. PI-PLC η2, expressed in the brain after birth, is a key enzyme in cellular calcium mobilization. In the brain, calcium plays a role in axon growth and retraction, growth cone guidance, synapse formation, and responses to various neurotransmitters. For its role in the nervous system, PI-PLC η2 might be a putative candidate gene for the neurodevelopmental delay observed in patients bearing 1p36.3 deletions.

2010 - Expression Pattern and Sub-Cellular Distribution of Phosphoinositide Specific Phospholipase C Enzymes After Treatment With U-73122 in Rat Astrocytoma Cells [Articolo su rivista]
LO VASCO, VINCENZA RITA; Fabrizi, Cinzia; Panetta, Barbara; Fumagalli, Lorenzo; Cocco, Lucio
abstract

Phosphoinositidc specific. phospholipase C (PI-PLC) enzymes interfere with the metabolism of inositol phospholipids (PI), molecules involved in signal transduction, a complex process depending on various components. Many evidences support the hypothesis that, in the glia, isoforms of PI-PLC family display different expression and/or sub cellular distribution under non-physiological conditions such as the rat astrocytes activation during neurodegeneration, the tumoural progression of some neoplasms and the inflammatory cascade activation after lipopolysaccharide administration, even if their role remains not completely elucidated. Treatment of a cultured established glioma cell line (C6 rat astrocytoma cell line) induces a modification in the pattern of expression and of sub cellular distribution of PI-PLCs compared to untreated cells. Special attention require PI-PLC beta3 and PI-PLC gamma2 isoforms, whose expression and sub cellular localization significantly differ after U-73122 treatment. The meaning of these modifications is unclear, also because the use of this N-aminosteroid compound remains controversial, inasmuch it has further actions which might contribute to the global effect recorded on the treated cells. J. Cell. Biochem. 110: 1005-1012, 2010. (C) 2010 Wiley-Liss, Inc.

2010 - Expression of phosphoinositide-specific phospholipase C isoenzymes in cultured astrocytes activated after stimulation with lipopolysaccharide [Articolo su rivista]
LO VASCO, VINCENZA RITA; Fabrizi, Cinzia; Fumagalli, Lorenzo; Cocco, L.
abstract

Signal transduction pathways, involved in cell cycle and activities, depend on various components including lipid signalling molecules, such as phosphoinositides and related enzymes. Many evidences support the hypothesis that inositol lipid cycle is involved in astrocytes activation during neurodegeneration. Previous studies investigated the pattern of expression of phosphoinositide-specific phospholipase C (PI-PLC) family isoforms in astrocytes, individuating in cultured neonatal rat astrocytes, supposed to be quiescent cells, the absence of some isoforms, accordingly to their well known tissue specificity. The same study was conducted in cultured rat astrocytoma C6 cells and designed a different pattern of expression of PI-PLCs in the neoplastic counterpart, accordingly to literature suggesting a PI signalling involvement in tumour progression. It is not clear the role of PI-PLC isoforms in inflammation; recent data demonstrate they are involved in cytokines production, with special regard to IL-6. PI-PLCs expression in LPS treated neonatal rat astrocytes performed by using RT-PCR, observed at 3, 6, 18 and 24 h intervals, expressed: PI-PLC beta 1, beta4 and gamma1 in all intervals analysed; PI-PLC delta1 at 6, 18 and 24 h; PI-PLC delta3 at 6 h after treatment. PI-PLC beta1, delta4 and epsilon, present in untreated astrocytes, were not detected after LPS treatment. Immunocytochemical analysis, performed to visualize the sub-cellular distribution of the expressed isoforms, demonstrated different patterns of localisation at different times of exposure. These observations suggest that PI-PLCs expression and distribution may play a role in ongoing inflammation process of CNS. J. Cell. Biochem. 109: 1006-1012, 2010. (C) 2010 Wiley-Liss, Inc.

2010 - Signalling in the genomic era [Articolo su rivista]
LO VASCO, VINCENZA RITA
abstract

For a complex organism, short range signalling is not sufficient to coordinate the behaviour of all cells composing itself. The response to stimuli is the reprogramming of cell activity (resulting in differentiation, proliferation, stand by or apoptosis depending on the set of signals). Cells own elaborate and complex systems of proteins that enable them to communicate, including both secreted signalling molecules and related factors, deriving from relic mechanisms. The intra and intercellular signalling are actively studied not only to comprehend the basic mechanisms that allowed the evolution of mammals species on earth, but also because the alteration of one or more of these pathways is recognized to be involved in a crescent number of human diseases, both degenerative and tumoural. That is, a growing body of evidences suggest that every human disease may be analyzed and classified by a "signalling disease" point of view. This approach opens new therapeutic perspectives, virtually amplifying for every single disease the number of therapeutic targets (in terms of both genes and proteins) to upstream and/or downstream, short and/or long distance proteins interacting with the altered molecule, thus individuating many other targets to which act upon.

2008 - Immunohistochemical profile of various neurotransmitters, neurotrophins and MIB-1 in cholesteatomas of the petrous bone [Articolo su rivista]
Artico, Marco; Bronzetti, Elena; LO VASCO, VINCENZA RITA; Ionta, Brunella; Alicino, Valentina; D'Ambrosio, A.; Magliulo, Giuseppe
abstract

Compared to the normal epidermal epithelium, cholesteatomas have altered growth properties characterized by the excessive growth of keratinocytes leading to mucosal destruction. Either congenital or acquired, these lesions, which grow in the middle ear space, the petrous apex or the mastoid of temporal bones, are mostly considered benign skin tumoral lesions. However, many questions remain concerning their pathophysiology. Numerous studies have been proposed to identify those cholesteatoma lesions at risk of recurrence, a possible event that may cause hearing loss. We examined patients with petrous apex or mastoid cholesteatoma in order to analyze the expression of various neurotransmitters, neurotrophins and their receptors and the Ki-67 antigen for identification of a possible relationship between clinical outcome and histopathological behaviour in terms of the proliferative activity of cholesteatomas. Expression of the analyzed molecules was studied using immunohistochemical methods in seven adult patients with petrous apex cholesteatoma who underwent surgical removal of the lesion. Our results, in accordance with published data, confirm that Molecular Immunology Borstel-1 (MIB-1) and certain neurotransmitters could be useful in the prognostic evaluation of the risk of recurrence of aggressive forms of cholesteatoma.

2007 - Expression of phosphoinositide-specific phospholipase C isoenzymes in cultured astrocytes [Articolo su rivista]
Lo Vasco, Vincenza Rita; Fabrizi, Cinzia; Artico, Marco; Cocco, Lucio; Maria Billi, Anna; Fumagalli, Lorenzo; Antonio Manzoli, Francesco
abstract

Signal transduction from plasma membrane to cell nucleus is a complex process depending on various components including lipid signaling molecules, in particular phosphoinositides and their related enzymes, which act at cell periphery and/or plasma membrane as well as at nuclear level. As far as the nervous system may concern the inositol lipid cycle has been hypothesized to be involved in numerous neural as well as glial functions. In this context, however, a precise panel of glial PLC isoforms has not been determined yet. In the present experiments we investigated astrocytic PLC isoforms in astrocytes obtained from foetal primary cultures of rat brain and from an established cultured (C6) rat astrocytoma cell line, two well known cell models for experimental studies on glia. Identification of PLC isoforms was achieved by using a combination of RT-PCR and immunocytochemistry experiments. While in both cell models the most represented PI-PLC isoforms were beta 4, gamma 1, delta 4, and epsilon, isoforms PI-PLC beta 2 and delta 3 were not detected. Moreover, in primary astrocyte cultures PI-PLC delta 3 resulted well expressed in C6 cells but was absent in astrocytes. Immunocytochemistry performed with antibodies against specific PLC isoforms substantially confirmed this pattern of expression both in astrocytes and C6 glioma cells. in particular while some isoenzymes (namely isoforms 133 and 134) resulted mainly nuclear, others (isoforms delta 4 and epsilon) were preferentially localized at cytoplasmic and plasma membrane level.

2006 - EXPRESSION OF PHOSPHOINOSITIDE SPECIFIC PHOSPHOLIPASE C IN CULTURED ACTIVATED ASTROCYTES [Relazione in Atti di Convegno]
LO VASCO, VINCENZA RITA; Fabrizi, Cinzia; Fumagalli, L.
abstract

2006 - Reply to Herens et al [Articolo su rivista]
LO VASCO, VINCENZA RITA; Follo, M; Cocco, L
abstract

2006 - Reply to Herens et al. [Articolo su rivista]
Lo Vasco, V. R.; Follo, M. Y.; Cocco, L.
abstract

2005 - Expression of neurotransmitters and neurotrophins in human adenoid tissue [Articolo su rivista]
Bronzetti, Elena; Artico, Marco; LO VASCO, VINCENZA RITA; Maria Felici, Laura; Bosco, Sandro; Magliulo, G.; Pompili, Elena; Fumagalli, Lorenzo
abstract

Mucosae-associated lymphoid tissues are richly innervated and the mucosae contain peptidergic nerve endings associated with different types of cells and macrophages. The lymphatic tissue is known to interact with the nervous system and several organs, implicated in the host response to a wide range of stressors, and is also richly innervated. We focussed our attention on the immune organs with particular regard to the human adenoid lymphatic tissues in order to investigate the neuroimmune links and the possible existence of relationships among different neurotransmitters and lymphocytes, macrophages, epithelial cells and nerve fibers by testing the expression of certain neurotransmitters and neurotrophins (NTs) with their own receptors.

2005 - Expression of phosphoinositide phospholipase C isoenzymes in cultured astrocytes [Relazione in Atti di Convegno]
LO VASCO, V.; Fabrizi, Cinzia; ARTICO AND LORENZO FUMAGALLI, Marco
abstract

2005 - Immunohistochemical profile of neurotransmitters and neurotrophin in human palatine tonsils [Relazione in Atti di Convegno]
Bronzetti, E; Artico, M; LO VASCO, VINCENZA RITA; Felici, Lm; Pignone, D; Magliulo, G.
abstract

2005 - Neurotransmitter and neurotrophin expression in human nasopharyngeal and palatine tonsils [Abstract in Atti di Convegno]
Bronzetti, E; Artico, M; E., Pompili; LO VASCO, VINCENZA RITA; Felici, Lm; Magliulo, G; Bosco, S; Vignone, D; Fumagalli, L
abstract

2005 - PLCB1 (phospholipase C, beta 1 (phosphoinositide-specific) [Curatela]
My, Follo; LO VASCO, V.; G, Martinelli; G, Palka; L, Cocco
abstract

2004 - ANALISI DEL RUOLO DEL GENE PI-PLC beta1 NELLA PROGRESSIONE DELLA SINDROME MIELODISPLASTICA IN LEUCEMIA MIELOIDE ACUTA [Working paper]
Lo Vasco, Vr.
abstract

2004 - Expression of phospholipase C beta family isoenzymes in C2C12 myoblasts during terminal differentiation [Articolo su rivista]
Faenza, Irene; Bavelloni, Alberto; Fiume, Roberta; Santi, Patrizia; Martelli, Alberto M.; Billi, A. M.; LO VASCO, VINCENZA RITA; Manzoli, Lucia; Cocco, Lucio
abstract

In the present work, we have analyzed the expression and subcellular localization of all the members of inositide-specific phospholipase C (PLCβ) family in muscle differentiation, given that nuclear PLCβ1 has been shown to be related to the differentiative process. Cell cultures of C2C12 myoblasts were induced to differentiate towards the phenotype of myotubes, which are also indicated as differentiated C2C12 cells. By means of immunochemical and immunocytochemical analysis, the expression and subcellular localization of PLCβ1, β2, β3, β4 have been assessed. As further characterization, we investigated the localization of PLCβ isoenzymes in C2C12 cells by fusing their cDNA to enhanced green fluorescent protein (GFP). In myoblast culture, PLCβ4 was the most expressed isoform in the cytoplasm, whereas PLCβ1 and β3 exhibited a lesser expression in this cell compartment. In nuclei of differentiated myotube culture, PLCβ1, isoform was expressed at the highest extent. A marked decrease of PLCβ4 expression in the cytoplasm of differentiated C2C12 cells was detected as compared to myoblasts. No relevant differences were evidenced as regards the expression of PLCβ3 at both cytoplasmatic and nuclear level, whilst PLCβ2 expression was almost undetactable. Therefore, we propose that the different subcellular expression of these PLC isoforms, namely the increase of nuclear PLCβ1 and the decrease of cytoplasmatic PLCβ4, during the establishment of myotube differentiation, is related to a spatial-temporal signaling event, involved in myogenic differentiation. Once again the subcellular localization appears to be a key step for the diverse signaling activity of PLCβs. © 2004 Wiley-Liss, Inc.

2004 - IMMUNOHISTOCHEMICAL PROFILE OF NEUROTRANSMITTERS AND NEUROTROPHINS IN HUMAN ADENOID TISSUE [Relazione in Atti di Convegno]
Bronzetti, E; Artico, M; LO VASCO, VINCENZA RITA; Felici, Lm; Bosco, S; Magliulo, Giuseppe; Vignone, D; Fumagalli, L.
abstract

2004 - Inositide-specific Phospholipase C beta1 gene deletion in the progression of Myelodisplastic Syndrome to Acute Myeloid Leukemia [Articolo su rivista]
LO VASCO, V.; G, Calabrese; L, Manzoli; Gd, Palka; A, Spadano; E, Morizio; P, Guancialifranchi; D, Fantasia; L, Cocco
abstract

Myelodysplastic syndrome (MDS) is an adult hematological disease that evolves into acute myeloid leukemia (AML) in about 30% of the cases. The availability of a highly specific probe moved us to perform in patients affected with MDS/AML, associated with normal karyotype, painting and fluorescence in situ hybridization (FISH) analysis aimed to check the inositide-specific phospholipase C (PI-PLC) beta1 gene, a player in the control of some checkpoints of the cell cycle. Here we present a preliminary observation in which FISH analysis disclosed in a small group of MDS/AML patients with normal karyotype the monoallelic deletion of the PI-PLCbeta1 gene. On the contrary, PI-PLC beta4, another gene coding for a signaling molecule, located on 20p12.3 at a distance as far as less than 1Mb from PI-PLCbeta1, is unaffected in MDS patients with the deletion of PI-PLC beta1 gene, hinting at an interstitial deletion. The MDS patients, bearing the deletion, rapidly evolved to AML. The data suggest the possible involvement of PI-PLCbeta1 in the progression of the disease and pave the way for a larger investigation aimed at identifying a possible high-risk group among MDS patients with a normal karyotype

2004 - Peribronchial innervation of the rat lung [Articolo su rivista]
Artico, Marco; Bosco, Sandro; Bronzetti, Elena; Maria Felici, Laura; Pelusi, G.; LO VASCO, VINCENZA RITA; Vitale, M.
abstract

Mammalian peribronchial tissue is supplied by several peptide-containing nerve fibers. Although it is well established that different neuropeptides exert significant effects on bronchial and vascular tone in the lungs, the role played by some neuromediators on the general regulation, differentiation and release of locally active substances is still controversial. We studied the innervation of rat peribronchial tissue by immunohistochemical techniques. The immunoperoxidase method with nickel amplification was applied to detect the distribution of nerve fibers using antibodies against the general neuronal marker PGP 9.5 (neuron-specific cytoplasmic protein), while the cholinacetyltransferase immunoreactivity was studied by immunohistochemistry. A slight immunoreactivity for NT receptors is observed in lung bronchial epithelium. There is increasing evidence that NTs may act with a paracrine mechanism regulating functional activity of neuronal and non-neuronal structures. A specific immunoreactivity for NTs and NT receptors was also demonstrated within different layers of large, medium and small sized intrapulmonary arteries and veins, according to a recent study of our group. Moreover our data describe the expression of NTs and NT receptors in lymphoid aggregates of the lung (BALT) in which both lymphocytes and macrophages express TrkA receptor and synthesize NTs. Our results show the presence of an extensive network of innervation in the rat peribronchial tissue, confirming a morphological basis for a possible neural modulation of the respiratory mucosa and the physiological/pathophysiologicaI mechanisms of the lung.

2003 - Analisi immunocitochimica e immunochimica dell'espressione delle isoforme della fosfolipasi C beta nel differenziamento muscolare [Relazione in Atti di Convegno]
Fiume, R.; Faenza, I.; Bavelloni, A.; LO VASCO, V.; Matteucci, S.; Cocco, L.; Manzoli, F. A.
abstract

2003 - Deletion of PI-PLC beta1 gene in myelodisplastic syndrome and acute myeloid leukemia [Relazione in Atti di Convegno]
LO VASCO, V.; Calabrese, G.; Palka, G.; Matteucci, A.; Fiume, R.; Cocco, L.
abstract

2003 - Immunohistochemical localization of PGP9.5, chat and neurotrophins in rat peribronchial lymphoid tissue [Relazione in Atti di Convegno]
E, Bronzetti; L, Felici; LO VASCO, V.; B, Panetta; B, Bronzetti
abstract

2002 - Nuclear localization of inositide signalling: role of PLCbeta1 in the expression of erythroid-specific transcription factors [Relazione in Atti di Convegno]
I, Faenza; A, Matteucci; Am, Billi; LO VASCO, V.; L, Manzoli
abstract

2002 - Pure 6p22-pter trisomic patient: Refined FISH characterization and genotype-phenotype correlation [Articolo su rivista]
Giardino, D.; Finelli, P.; Caufin, D.; Gottardi, G.; Lo Vasco, Vincenza Rita; Turolla, L.; Larizza, L.
abstract

First described in 1971, partial trisomy 6p is uncommon and generally secondary to a familial reciprocal translocation. The proximal breakpoint of the reported cases varies from p11 to p25. We here report on a patient with moderate mental retardation, craniofacial and pigmentary anomalies, proteinuria, and hyperglycemia who was found to have a mosaic karyotype 46,X,add (Y)(q12)/45,X. Fluorescence in situ hybridization (FISH) enabled us to identify that the additional material on Yqh derived from 6p and to define the rearrangement as der(Y)t(Y;6)(q12;p22). To the best of our knowledge, this is the first case of trisomy 6p22-pter without an associated deleted segment; the second breakpoint of the rearrangement is in Yqh. Precise mapping of the centromeric breakpoint of the trisomic 6p segment allowed a more convincing correlation between partial 6p trisomy and clinical phenotype to be addressed. In particular, the proteinuria often observed in 6p trisomic patients could be assigned to the 6p22-6pter region. (C) 2002 Wiley-Liss, Inc.

2000 - Un caso di associazione VACTERL con pancreas anulare [Relazione in Atti di Convegno]
LO VASCO, V.; L, Turolla; E, Frate
abstract

1999 - Three years experience in Down Syndrome Screening [Relazione in Atti di Convegno]
L, Caberlotto; L, Turolla; M, Meneghel; LO VASCO, V.
abstract

1999 - Un caso di canale atrioventricolare con delezione del cromosoma 8(p11.21-p21.1) [Relazione in Atti di Convegno]
V, Cusin; E, Frate; LO VASCO, V.; L, Santarini; L, Turolla
abstract

1999 - Un caso di sbilanciamento di traslocazione 11;22 familiare con ipoacusia neurosensoriale [Relazione in Atti di Convegno]
LO VASCO, V.; L, Bortotto; E, Frate; V, Cusin; L, Turolla
abstract

1999 - Un caso di sindrome Trico-Dento-Ossea [Relazione in Atti di Convegno]
E, Frate; V, Cusin; LO VASCO, V.; S, Sivolella; L, Turolla
abstract

1994 - AMNIOTIC BAND SEQUENCE IN CHILD OF THALIDOMIDE VICTIM [Articolo su rivista]
Tenconi, R.; Clementi, M.; Notari, L.; LO VASCO, VINCENZA RITA
abstract