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ELEONORA CATTIN

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Dipartimento di Scienze della Vita

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Pubblicazioni

2022 - CRISPR/Cas9 Allele-Specific Design To Inactivate A Dominant-Negative Mutation In COL6A1 Causing Ullrich Muscular Dystrophy [Abstract in Atti di Convegno]
Benati, Daniela; Patrizi, Clarissa; Cattin, Eleonora; Ferrari, Tommaso; Pedrazzoli, Eleonora; Marchionni, Matteo; Rossi, Rachele; D’Amico, Adele; Merlini, Luciano; Sabatelli, Patrizia; Ferlini, Alessandra; Gualandi, Francesca; Recchia, Alessandra
abstract

2022 - CRISPR/Cas9 allele-specifc design to inactivate a dominant-negative mutation in COL6A1 causing Ullrich muscular dystrophy. [Abstract in Atti di Convegno]
Benati, Daniela; Patrizi, Clarissa; Cattin, Eleonora; Ferrari, Tommaso; Pedrazzoli, Eleonora; Marchionni, Matteo; Rossi, Rachele; D'Amico, Adele; Merlini, Luciano; Sabatelli, Patrizia; Ferlini, Alessandra; Gualandi, Francesca; Recchia, Alessandra.
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2022 - Engineered Sleeping Beauty Transposon as Efficient System to Optimize Chimp Adenoviral Production [Articolo su rivista]
Baldassarri, S.; Benati, D.; D'Alessio, F.; Patrizi, C.; Cattin, E.; Gentile, M.; Raggioli, A.; Recchia, A.
abstract

Sleeping Beauty (SB) is the first DNA transposon employed for efficient transposition in vertebrate cells, opening new applications for genetic engineering and gene therapies. A transposon-based gene delivery system holds the favourable features of non-viral vectors and an attractive safety profile. Here, we employed SB to engineer HEK293 cells for optimizing the production of a chimpanzee Adenovector (chAd) belonging to the Human Mastadenovirus C species. To date, chAd vectors are employed in several clinical settings for infectious diseases, last but not least COVID-19. A robust, efficient and quick viral vector production could advance the clinical application of chAd vectors. To this aim, we firstly swapped the hAd5 E1 with chAd-C E1 gene by using the CRISPR/Cas9 system. We demonstrated that in the absence of human Ad5 E1, chimp Ad-C E1 gene did not support HEK293 survival. To improve chAd-C vector production, we engineered HEK293 cells to stably express the chAd-C precursor terminal protein (ch.pTP), which plays a crucial role in chimpanzee Adenoviral DNA replication. The results indicate that exogenous ch.pTP expression significantly ameliorate the packaging and amplification of recombinant chAd-C vectors thus, the engineered HEK293ch.pTP cells could represent a superior packaging cell line for the production of these vectors.

2021 - CRISPR-mediated genome editing to redirect T cells against Non-Small Cell Lung Cancer [Abstract in Atti di Convegno]
Benati, Daniela; Masciale, Valentina; Grisendi, Giulia; Ferrari, Tommaso; Cattin, Eleonora; Marchionni, Matteo; Aramini, Beatrice; Dominici, Massimo; Recchia, Alessandra
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2021 - CRISPR-mediated genome editing to redirect T cells against Non-Small Cell Lung Cancer [Abstract in Atti di Convegno]
Benati, Daniela; Masciale, Valentina; Grisendi, Giulia; Ferrari, Tommaso; Cattin, Eleonora; Aramini, Beatrice; Dominici, Massimo; Recchia, Alessandra
abstract